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Record Information
Version5.0
StatusExpected but not Quantified
Creation Date2006-05-22 15:12:25 UTC
Update Date2022-03-07 02:49:17 UTC
HMDB IDHMDB0002995
Secondary Accession Numbers
  • HMDB02995
Metabolite Identification
Common Name12-Keto-tetrahydro-leukotriene B4
Description12-keto-tetrahydro-Leukotriene B4 is an inactivated enzymatic metabolite of leukotriene B4(LTB4), product of the human liver enzyme leukotriene B4 (LTB4) 12-hydroxydehydrogenase, also found in the porcine kidney and other mammals. LTB4 is the major metabolite in neutrophil polymorphonuclear leukocytes. Leukotrienes are metabolites of arachidonic acid derived from the action of 5-LO (5-lipoxygenase). The immediate product of 5-LO is LTA4 (leukotriene A4), which is enzymatically converted into either LTB4 (leukotriene B4) by LTA4 hydrolase or LTC4 (leukotriene C4) by LTC4 synthase. The regulation of leukotriene production occurs at various levels, including expression of 5-LO, translocation of 5-LO to the perinuclear region and phosphorylation to either enhance or inhibit the activity of 5-LO. Biologically active LTB4 is metabolized by w-oxidation carried out by specific cytochrome P450s (CYP4F) followed by beta-oxidation from the w-carboxy position and after CoA ester formation. (PMID: 17623009 , 8394361 , 9667737 )Leukotrienes are eicosanoids. The eicosanoids consist of the prostaglandins (PGs), thromboxanes (TXs), leukotrienes (LTs), and lipoxins (LXs). The PGs and TXs are collectively identified as prostanoids. Prostaglandins were originally shown to be synthesized in the prostate gland, thromboxanes from platelets (thrombocytes), and leukotrienes from leukocytes, hence the derivation of their names. All mammalian cells except erythrocytes synthesize eicosanoids. These molecules are extremely potent, able to cause profound physiological effects at very dilute concentrations. All eicosanoids function locally at the site of synthesis, through receptor-mediated G-protein linked signalling pathways.
Structure
Data?1582752260
Synonyms
ValueSource
12-Keto-10,11,14,15-tetrahydro-LTB4HMDB
5S-Hydroxy-12-keto-6Z,8E-eicosadienoateHMDB
5S-Hydroxy-12-keto-6Z,8E-eicosadienoic acidHMDB
Chemical FormulaC20H34O4
Average Molecular Weight338.4816
Monoisotopic Molecular Weight338.245709576
IUPAC Name(5S,6Z,8E)-5-hydroxy-12-oxoicosa-6,8-dienoic acid
Traditional Nameleukotriene B
CAS Registry NumberNot Available
SMILES
CCCCCCCCC(=O)CC\C=C\C=C/[C@@H](O)CCCC(O)=O
InChI Identifier
InChI=1S/C20H34O4/c1-2-3-4-5-6-9-13-18(21)14-10-7-8-11-15-19(22)16-12-17-20(23)24/h7-8,11,15,19,22H,2-6,9-10,12-14,16-17H2,1H3,(H,23,24)/b8-7+,15-11-/t19-/m1/s1
InChI KeyRRTYEHFGQWNQKK-KIQAWMAPSA-N
Chemical Taxonomy
Description Belongs to the class of organic compounds known as other hydroxyeicosapolyenoic acids. These are hydroxyeicosapolyenoic acids which do not belong to the Hydroxyeicosapentaenoic acids, the Hydroxyeicosatetraenoic acids, or the Hydroxyeicosatrienoic acids.
KingdomOrganic compounds
Super ClassLipids and lipid-like molecules
ClassFatty Acyls
Sub ClassEicosanoids
Direct ParentOther hydroxyeicosapolyenoic acids
Alternative Parents
Substituents
  • Hydroxyeicosapolyenoic acid
  • Long-chain fatty acid
  • Hydroxy fatty acid
  • Keto fatty acid
  • Fatty acid
  • Unsaturated fatty acid
  • Ketone
  • Secondary alcohol
  • Carboxylic acid derivative
  • Carboxylic acid
  • Monocarboxylic acid or derivatives
  • Organic oxide
  • Organic oxygen compound
  • Alcohol
  • Hydrocarbon derivative
  • Organooxygen compound
  • Carbonyl group
  • Aliphatic acyclic compound
Molecular FrameworkAliphatic acyclic compounds
External Descriptors
Ontology
Physiological effectNot Available
Disposition
ProcessNot Available
RoleNot Available
Physical Properties
StateSolid
Experimental Molecular Properties
PropertyValueReference
Melting PointNot AvailableNot Available
Boiling PointNot AvailableNot Available
Water SolubilityNot AvailableNot Available
LogP3.58Not Available
Experimental Chromatographic PropertiesNot Available
Predicted Molecular Properties
PropertyValueSource
Water Solubility0.0067 g/LALOGPS
logP4.96ALOGPS
logP4.9ChemAxon
logS-4.7ALOGPS
pKa (Strongest Acidic)4.58ChemAxon
pKa (Strongest Basic)-1.5ChemAxon
Physiological Charge-1ChemAxon
Hydrogen Acceptor Count4ChemAxon
Hydrogen Donor Count2ChemAxon
Polar Surface Area74.6 ŲChemAxon
Rotatable Bond Count16ChemAxon
Refractivity99.88 m³·mol⁻¹ChemAxon
Polarizability41.03 ųChemAxon
Number of Rings0ChemAxon
BioavailabilityYesChemAxon
Rule of FiveYesChemAxon
Ghose FilterYesChemAxon
Veber's RuleNoChemAxon
MDDR-like RuleNoChemAxon
Predicted Chromatographic Properties

Predicted Collision Cross Sections

PredictorAdduct TypeCCS Value (Å2)Reference
DarkChem[M+H]+190.57931661259
DarkChem[M-H]-189.79231661259
DeepCCS[M+H]+197.8730932474
DeepCCS[M-H]-195.51230932474
DeepCCS[M-2H]-228.39830932474
DeepCCS[M+Na]+203.96430932474
AllCCS[M+H]+192.132859911
AllCCS[M+H-H2O]+189.432859911
AllCCS[M+NH4]+194.632859911
AllCCS[M+Na]+195.332859911
AllCCS[M-H]-189.632859911
AllCCS[M+Na-2H]-191.532859911
AllCCS[M+HCOO]-193.732859911

Predicted Kovats Retention Indices

Underivatized

MetaboliteSMILESKovats RI ValueColumn TypeReference
12-Keto-tetrahydro-leukotriene B4CCCCCCCCC(=O)CC\C=C\C=C/[C@@H](O)CCCC(O)=O4382.9Standard polar33892256
12-Keto-tetrahydro-leukotriene B4CCCCCCCCC(=O)CC\C=C\C=C/[C@@H](O)CCCC(O)=O2574.3Standard non polar33892256
12-Keto-tetrahydro-leukotriene B4CCCCCCCCC(=O)CC\C=C\C=C/[C@@H](O)CCCC(O)=O2762.0Semi standard non polar33892256

Derivatized

Derivative Name / StructureSMILESKovats RI ValueColumn TypeReference
12-Keto-tetrahydro-leukotriene B4,1TMS,isomer #1CCCCCCCCC(=O)CC/C=C/C=C\[C@H](CCCC(=O)O)O[Si](C)(C)C2881.7Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,1TMS,isomer #2CCCCCCCCC(=O)CC/C=C/C=C\[C@@H](O)CCCC(=O)O[Si](C)(C)C2838.9Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,1TMS,isomer #3CCCCCCCCC(=CC/C=C/C=C\[C@@H](O)CCCC(=O)O)O[Si](C)(C)C2984.7Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,1TMS,isomer #4CCCCCCCC=C(CC/C=C/C=C\[C@@H](O)CCCC(=O)O)O[Si](C)(C)C2978.7Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TMS,isomer #1CCCCCCCCC(=O)CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C2867.6Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TMS,isomer #2CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O)O[Si](C)(C)C)O[Si](C)(C)C2998.6Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TMS,isomer #3CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O)O[Si](C)(C)C)O[Si](C)(C)C2997.2Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TMS,isomer #4CCCCCCCCC(=CC/C=C/C=C\[C@@H](O)CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C2934.8Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TMS,isomer #5CCCCCCCC=C(CC/C=C/C=C\[C@@H](O)CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C2930.3Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TMS,isomer #1CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C)O[Si](C)(C)C2959.0Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TMS,isomer #1CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C)O[Si](C)(C)C2788.6Standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TMS,isomer #1CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C)O[Si](C)(C)C2950.1Standard polar33892256
12-Keto-tetrahydro-leukotriene B4,3TMS,isomer #2CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C)O[Si](C)(C)C2947.2Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TMS,isomer #2CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C)O[Si](C)(C)C2775.0Standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TMS,isomer #2CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C)O[Si](C)(C)C)O[Si](C)(C)C2912.1Standard polar33892256
12-Keto-tetrahydro-leukotriene B4,1TBDMS,isomer #1CCCCCCCCC(=O)CC/C=C/C=C\[C@H](CCCC(=O)O)O[Si](C)(C)C(C)(C)C3135.2Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,1TBDMS,isomer #2CCCCCCCCC(=O)CC/C=C/C=C\[C@@H](O)CCCC(=O)O[Si](C)(C)C(C)(C)C3089.5Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,1TBDMS,isomer #3CCCCCCCCC(=CC/C=C/C=C\[C@@H](O)CCCC(=O)O)O[Si](C)(C)C(C)(C)C3233.0Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,1TBDMS,isomer #4CCCCCCCC=C(CC/C=C/C=C\[C@@H](O)CCCC(=O)O)O[Si](C)(C)C(C)(C)C3222.9Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TBDMS,isomer #1CCCCCCCCC(=O)CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3375.8Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TBDMS,isomer #2CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3498.3Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TBDMS,isomer #3CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3474.6Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TBDMS,isomer #4CCCCCCCCC(=CC/C=C/C=C\[C@@H](O)CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3438.6Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,2TBDMS,isomer #5CCCCCCCC=C(CC/C=C/C=C\[C@@H](O)CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3409.1Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TBDMS,isomer #1CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3732.2Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TBDMS,isomer #1CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3295.4Standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TBDMS,isomer #1CCCCCCCCC(=CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3118.0Standard polar33892256
12-Keto-tetrahydro-leukotriene B4,3TBDMS,isomer #2CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3690.7Semi standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TBDMS,isomer #2CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3275.5Standard non polar33892256
12-Keto-tetrahydro-leukotriene B4,3TBDMS,isomer #2CCCCCCCC=C(CC/C=C/C=C\[C@H](CCCC(=O)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C)O[Si](C)(C)C(C)(C)C3083.8Standard polar33892256
Spectra

GC-MS Spectra

Spectrum TypeDescriptionSplash KeyDeposition DateSourceView
Predicted GC-MSPredicted GC-MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 GC-MS (Non-derivatized) - 70eV, Positivesplash10-0pi4-8982000000-17057de07ae4d4492fc02017-09-01Wishart LabView Spectrum
Predicted GC-MSPredicted GC-MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 GC-MS (2 TMS) - 70eV, Positivesplash10-00xr-9125500000-270788ed45d1210a5ced2017-10-06Wishart LabView Spectrum
Predicted GC-MSPredicted GC-MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 GC-MS (Non-derivatized) - 70eV, PositiveNot Available2021-10-12Wishart LabView Spectrum
Predicted GC-MSPredicted GC-MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 GC-MS (Non-derivatized) - 70eV, PositiveNot Available2021-10-12Wishart LabView Spectrum

MS/MS Spectra

Spectrum TypeDescriptionSplash KeyDeposition DateSourceView
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 10V, Positive-QTOFsplash10-0fk9-0019000000-6fc13279cd371fea9b4a2017-09-01Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 20V, Positive-QTOFsplash10-0hbc-3595000000-77ffa7d42a1ed9c17ec02017-09-01Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 40V, Positive-QTOFsplash10-052f-9650000000-0117916eb757427250d12017-09-01Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 10V, Negative-QTOFsplash10-000i-0019000000-7581ef598d01fcad72b02017-09-01Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 20V, Negative-QTOFsplash10-0670-2569000000-4fe74a0ed76cc4e4ad6f2017-09-01Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 40V, Negative-QTOFsplash10-0a4i-9620000000-e99d688d13888f06c2402017-09-01Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 10V, Negative-QTOFsplash10-000i-0009000000-d3a39dea97cc623c601c2021-09-23Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 20V, Negative-QTOFsplash10-05n0-3539000000-db63e5403378997697fe2021-09-23Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 40V, Negative-QTOFsplash10-052f-9473000000-7f80e891c9d7434565a52021-09-23Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 10V, Positive-QTOFsplash10-0fk9-0119000000-3bfe6f6c002dc5f8d2642021-09-25Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 20V, Positive-QTOFsplash10-0uk9-2239000000-6f8702bad18e21c698a32021-09-25Wishart LabView Spectrum
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 12-Keto-tetrahydro-leukotriene B4 40V, Positive-QTOFsplash10-0a4i-9300000000-78bcb027810003a840362021-09-25Wishart LabView Spectrum
Biological Properties
Cellular Locations
  • Extracellular
  • Membrane (predicted from logP)
Biospecimen LocationsNot Available
Tissue LocationsNot Available
Pathways
Normal Concentrations
Not Available
Abnormal Concentrations
Not Available
Associated Disorders and Diseases
Disease ReferencesNone
Associated OMIM IDsNone
DrugBank IDNot Available
Phenol Explorer Compound IDNot Available
FooDB IDFDB023093
KNApSAcK IDNot Available
Chemspider ID4446246
KEGG Compound IDC02165
BioCyc IDNot Available
BiGG IDNot Available
Wikipedia LinkNot Available
METLIN IDNot Available
PubChem Compound5283122
PDB IDNot Available
ChEBI ID15647
Food Biomarker OntologyNot Available
VMH IDNot Available
MarkerDB IDNot Available
Good Scents IDNot Available
References
Synthesis ReferenceNot Available
Material Safety Data Sheet (MSDS)Not Available
General References
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  2. Iversen L, Fogh K, Ziboh VA, Kristensen P, Schmedes A, Kragballe K: Leukotriene B4 formation during human neutrophil keratinocyte interactions: evidence for transformation of leukotriene A4 by putative keratinocyte leukotriene A4 hydrolase. J Invest Dermatol. 1993 Mar;100(3):293-8. [PubMed:8382716 ]
  3. Mozalevskii AF, Travianko TD, Iakovlev AA, Smirnova EA, Novikova NP, Sapa IIu: [Content of arachidonic acid metabolites in blood and saliva of children with bronchial asthma]. Ukr Biokhim Zh (1978). 1997 Sep-Dec;69(5-6):162-8. [PubMed:9606840 ]
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  5. Zijlstra FJ, Wilson JH: 15-HETE is the main eicosanoid present in mucus of ulcerative proctocolitis. Prostaglandins Leukot Essent Fatty Acids. 1991 May;43(1):55-9. [PubMed:1652771 ]
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  10. Gambero A, Landucci EC, Toyama MH, Marangoni S, Giglio JR, Nader HB, Dietrich CP, De Nucci G, Antunes E: Human neutrophil migration in vitro induced by secretory phospholipases A2: a role for cell surface glycosaminoglycans. Biochem Pharmacol. 2002 Jan 1;63(1):65-72. [PubMed:11754875 ]
  11. Labaudiniere R, Hendel W, Terlain B, Cavy F, Marquis O, Dereu N: omega-[(4-Phenyl-2-quinolyl)oxy]alkanoic acid derivatives: a new family of potent LTB4 antagonists. J Med Chem. 1992 Nov 13;35(23):4306-14. [PubMed:1333011 ]
  12. Labaudiniere R, Dereu N, Cavy F, Guillet MC, Marquis O, Terlain B: omega-[(4,6-Diphenyl-2-pyridyl)oxy]alkanoic acid derivatives: a new family of potent and orally active LTB4 antagonists. J Med Chem. 1992 Nov 13;35(23):4315-24. [PubMed:1333012 ]
  13. Costello PB, Baer AN, Green FA: Lipoxygenase products in inflammatory synovial fluids and other exudates. Ann Rheum Dis. 1992 Nov;51(11):1215-8. [PubMed:1334643 ]
  14. Zsila F, Bikadi Z, Lockwood SF: In vitro binding of leukotriene B4 (LTB4) to human serum albumin: evidence from spectroscopic, molecular modeling, and competitive displacement studies. Bioorg Med Chem Lett. 2005 Aug 15;15(16):3725-31. [PubMed:15993588 ]
  15. Bentancur AG, Naveh N, Lancri J, Selah BA, Shtrasburg S, Livneh A: Urine leukotriene B4 in familial Mediterranean fever and other forms of right lower abdominal pain. Acad Emerg Med. 2005 Jul;12(7):671-4. [PubMed:15995103 ]
  16. Yan Y, Wang B, Zuo YG, Qu T: Inhibitory effects of mizolastine on ultraviolet B-induced leukotriene B4 production and 5-lipoxygenase expression in normal human dermal fibroblasts in vitro. Photochem Photobiol. 2006 May-Jun;82(3):665-9. [PubMed:16402861 ]
  17. Elgebaly SA, Donshik PC, Rahhal F, Williams W: Neutrophil chemotactic factors in the tears of giant papillary conjunctivitis patients. Invest Ophthalmol Vis Sci. 1991 Jan;32(1):208-13. [PubMed:1846131 ]
  18. Doyle MJ, Eichhold TH, Hynd BA, Weisman SM: Determination of leukotriene B4 in human plasma by gas chromatography using a mass selective detector and a stable isotope labelled internal standard. Effect of NE-11740 on arachidonic acid metabolism. J Pharm Biomed Anal. 1990;8(2):137-42. [PubMed:1965571 ]
  19. Fujii I, Shingu M, Nobunaga M: Monocyte activation in early onset rheumatoid arthritis. Ann Rheum Dis. 1990 Jul;49(7):497-503. [PubMed:2166487 ]
  20. Abayasekara DR, Kurlak LO, Jeremy JY, Dandona P, Sharpe RM, Cooke BA: The levels and possible involvement of leukotriene B4 and prostaglandin F2 alpha in the control of interstitial fluid volume in the rat testis. Int J Androl. 1990 Oct;13(5):408-18. [PubMed:2178154 ]
  21. Mukhtar H, Bik DP, Ruzicka T, Merk HF, Bickers DR: Cytochrome P-450-dependent omega-oxidation of leukotriene B4 in rodent and human epidermis. J Invest Dermatol. 1989 Aug;93(2):231-5. [PubMed:2474030 ]
  22. Merrill JE, Strom SR, Ellison GW, Myers LW: In vitro study of mediators of inflammation in multiple sclerosis. J Clin Immunol. 1989 Mar;9(2):84-96. [PubMed:2541163 ]
  23. Hughes H, Mitchell JR, Gaskell SJ: Quantitation of leukotriene B4 in human serum by negative ion gas chromatography-mass spectrometry. Anal Biochem. 1989 Jun;179(2):304-8. [PubMed:2549806 ]
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Enzymes

General function:
Involved in monooxygenase activity
Specific function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Gene Name:
CYP4F2
Uniprot ID:
P78329
Molecular weight:
59852.825
General function:
Involved in monooxygenase activity
Specific function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.
Gene Name:
CYP4F3
Uniprot ID:
Q08477
Molecular weight:
59846.085
General function:
Involved in binding
Specific function:
Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity.
Gene Name:
LTA4H
Uniprot ID:
P09960
Molecular weight:
69284.64