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Identification
HMDB Protein ID HMDBP00123
Secondary Accession Numbers
  • 5355
Name Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
Synonyms
  1. Polyamine oxidase
Gene Name PAOX
Protein Type Unknown
Biological Properties
General Function Amino acid transport and metabolism
Specific Function Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
Pathways
  • Peroxisome
  • spermine metabolism
Reactions
N1-Acetylspermine + Oxygen + Water → Spermidine + Acetamidopropanal + Hydrogen peroxide details
N1-Acetylspermidine + Oxygen + Water → Putrescine + Acetamidopropanal + Hydrogen peroxide details
N1,N12-Diacetylspermine + Oxygen + Water → N1-Acetylspermidine + 3-Acetamidobutanal + Hydrogen peroxide details
GO Classification
Biological Process
small molecule metabolic process
xenobiotic metabolic process
polyamine catabolic process
positive regulation of spermidine biosynthetic process
putrescine biosynthetic process
putrescine catabolic process
spermidine catabolic process
spermine catabolic process
Cellular Component
peroxisomal matrix
Molecular Function
N(1),N(12)-diacetylspermine:oxygen oxidoreductase (3-acetamidopropanal-forming) activity
N1-acetylspermidine:oxygen oxidoreductase (3-acetamidopropanal-forming) activity
N1-acetylspermine:oxygen oxidoreductase (3-acetamidopropanal-forming) activity
polyamine oxidase activity
spermidine:oxygen oxidoreductase (3-aminopropanal-forming) activity
spermine:oxygen oxidoreductase (spermidine-forming) activity
Cellular Location
  1. Cytoplasm
  2. Peroxisome
Gene Properties
Chromosome Location 10
Locus 10q26.3
SNPs PAOX
Gene Sequence
>1950 bp
ATGGAGTCGACCGGCAGCGTCGGGGAGGCCCCGGGCGGACCCCGGGTGCTGGTGGTGGGC
GGCGGCATCGCGGGGCTGGGCGCGGCGCAGAGGCTCTGCGGCCACTCCGCCTTCCCGCAC
CTACGGGTCCTGGAGGCCACGGCCCGTGCCGGGGGCCGCATCCGCTCGGAGCGCTGCTTC
GGTGGCGTGGTGGAGGTGGGCGCGCACTGGATCCATGGGCCCTCCCGGGGTAACCCCGTC
TTCCAGCTGGCTGCTGAGTACGGGCTGCTGGGGGAGAAGGAGCTGTCCCAGGAGAACCAG
CTGGTGGAGACCGGGGGTCACGTGGGCCTGCCCTCCGTGAGCTACGCCAGCTCCGGGACC
AGCGTGAGCCTCCAGCTGGTGGCGGAGATGGCGACTCTGTTCTACGGCCTGATAGACCAG
ACCCGGGAGTTCCTGCACGCTGCAGAGACCCCGGTGCCCAGCGTCGGGGAGTACCTCAAG
AAGGAGATTGGCCAGCACGTGGCGAGGCTCTGCGGCCACTCCGCCTTCCCACACCTGCGG
GTCCTGGAGGCCACGGCCCGCGCCGGGGGCCGCATCCGCTCGGAGCGCTGCTTCGGTGGC
GTGGTGGAGGTGGGCGCGCACTGGATCCATGGGCCCTCCCGGGGTAACCCCGTCTTCCAG
CTGGCTGCTGAGTACGGGCTGCTGGGGGAGAAGGAGCTGTCCCAGGAGAACCAGCTGGTG
GAGACCGGGGGTCACGTGGGCCTGCCCTCCGTGAGCTACGCCAGCTCCGGAGCCAGCGTG
AGCCTCCAGCTGGTGGCGGAGATGGCGACTCTGTTCTACGGCCTGATAGACCAGACCCGG
GAGTTCCTGCACGCTGCAGAGACCCCGGTGCCCAGCGTCGGGGAGTACCTCAAGAAGGAG
ATTGGCCAGCACGTGGCCGGCTGGACAGAGGATGAGGAGACCAGGAAGCTGAAGCTGGCC
GTCCTGAACTCCTTCTTCAACCTGGAATGCTGTGTGAGCGGCACCCACAGCATGGACCTG
GTGGCCCTGGCACCCTTTGGGGAGTATACCGTGCTGCCGGGGCTGGACTGCACCTTTTCT
AAGGGCTATCAAGGACTCACAAACTGCATGATGGCCGCCCTGCCGGAGGACACTGTAGTT
TTTGAGAAGCCTGTGAAGACCATCCACTGGAACGGGTCCTTCCAGGAGGCAGCCTTTCCC
GGGGAGACCTTTCCAGTGTCGGTAGAGTGTGAGGATGGAGACCGGTTCCCGGCGCACCAT
GTCATCGTCACCGTGCCCTTAGGTTTTCTTAGGGAACATTTGGACACCTTCTTTGACCCT
CCCCTGCCGGCTGAGAAGGCAGAAGCAATCAGGAAGATAGGCTTTGGGACCAACAACAAA
ATCTTCCTGGAGTTTGAGGAGCCCTTCTGGGAGCCAGACTGCCAGCTGATCCAGCTGGTG
TGGGAGGACACGTCGCCCCTGGAGGATGCTGCCCCTGAGCTACAGGACGCCTGGTTCCGG
AAGCTCATTGGCTTTGTGGTCCTGCCTGCCTTTGCGTCTGTCCACGTTCTCTGTGGGTTC
ATTGCCGGACTTGAGTCTGAGTTCATGGAGACTCTGTCGGATGAAGAAGTACTTCTGTGT
CTCACCCAAGTGCTCCGGAGAGTGACAGGAAACCCACGGCTCCCCGCGCCCAAGAGCGTC
CTGCGGTCTCGCTGGCACAGCGCCCCGTACACTAGGGGGTCCTACAGCTACGTGGCCGTG
GGCAGTACTGGGGGCGACCTGGACCTGCTGGCTCAGCCCCTCCCTGCAGACGGCGCCGGC
GCCCAGCTCCAGATCCTGTTTGCGGGGGAAGCCACACATCGCACGTTTTACTCCACGACG
CACGGGGCTCTGCTGTCGGGATGGAGGGAGGCCGACCGCCTCCTCAGTCTGTGGGCCCCG
CAGGTGCAGCAGCCCAGGCCGAGGCTCTAG
Protein Properties
Number of Residues 649
Molecular Weight 55512.64
Theoretical pI 5.233
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
MESTGSVGEAPGGPRVLVVGGGIAGLGAAQRLCGHSAFPHLRVLEATARAGGRIRSERCF
GGVVEVGAHWIHGPSRGNPVFQLAAEYGLLGEKELSQENQLVETGGHVGLPSVSYASSGT
SVSLQLVAEMATLFYGLIDQTREFLHAAETPVPSVGEYLKKEIGQHVARLCGHSAFPHLR
VLEATARAGGRIRSERCFGGVVEVGAHWIHGPSRGNPVFQLAAEYGLLGEKELSQENQLV
ETGGHVGLPSVSYASSGASVSLQLVAEMATLFYGLIDQTREFLHAAETPVPSVGEYLKKE
IGQHVAGWTEDEETRKLKLAVLNSFFNLECCVSGTHSMDLVALAPFGEYTVLPGLDCTFS
KGYQGLTNCMMAALPEDTVVFEKPVKTIHWNGSFQEAAFPGETFPVSVECEDGDRFPAHH
VIVTVPLGFLREHLDTFFDPPLPAEKAEAIRKIGFGTNNKIFLEFEEPFWEPDCQLIQLV
WEDTSPLEDAAPELQDAWFRKLIGFVVLPAFASVHVLCGFIAGLESEFMETLSDEEVLLC
LTQVLRRVTGNPRLPAPKSVLRSRWHSAPYTRGSYSYVAVGSTGGDLDLLAQPLPADGAG
AQLQILFAGEATHRTFYSTTHGALLSGWREADRLLSLWAPQVQQPRPRL
GenBank ID Protein 45439842
UniProtKB/Swiss-Prot ID Q6QHF9
UniProtKB/Swiss-Prot Entry Name PAOX_HUMAN
PDB IDs Not Available
GenBank Gene ID AY541520
GeneCard ID PAOX
GenAtlas ID PAOX
HGNC ID HGNC:20837
References
General References
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