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Identification
HMDB Protein ID HMDBP00165
Secondary Accession Numbers
  • 5397
Name Dimethylaniline monooxygenase [N-oxide-forming] 4
Synonyms
  1. Dimethylaniline oxidase 4
  2. FMO 4
  3. Hepatic flavin-containing monooxygenase 4
Gene Name FMO4
Protein Type Unknown
Biological Properties
General Function Involved in flavin-containing monooxygenase activity
Specific Function This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides.
Pathways
  • Drug metabolism - cytochrome P450
Reactions
N,N-Dimethylaniline + NADPH + Oxygen → Dimethylaniline-N-oxide + NADP + Water details
Trimethylamine + NADPH + Hydrogen Ion + Oxygen → Trimethylamine N-oxide + NADP + Water details
Tamoxifen + Oxygen + NADPH + Hydrogen Ion → Tamoxifen N-oxide + NADP + Water details
GO Classification
Biological Process
xenobiotic metabolic process
Cellular Component
intrinsic to endoplasmic reticulum membrane
intracellular membrane-bounded organelle
integral to membrane
Component
cell part
membrane part
intrinsic to membrane
intrinsic to organelle membrane
intrinsic to endoplasmic reticulum membrane
Function
binding
nucleotide binding
catalytic activity
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
nadp or nadph binding
monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, nadh or nadph as one donor, and incorporation of one atom of oxygen
flavin-containing monooxygenase activity
oxidoreductase activity
fad or fadh2 binding
Molecular Function
NADP binding
flavin adenine dinucleotide binding
N,N-dimethylaniline monooxygenase activity
Process
metabolic process
oxidation reduction
Cellular Location
  1. Endoplasmic reticulum membrane
  2. Microsome membrane
Gene Properties
Chromosome Location 1
Locus 1q24.3
SNPs FMO4
Gene Sequence
>1677 bp
ATGGCCAAGAAAGTTGCAGTGATTGGAGCTGGTGTGAGTGGCCTCTCCTCCATCAAATGC
TGTGTGGATGAGGACCTGGAGCCCACCTGCTTTGAGAGAAGTGATGACATTGGGGGATTA
TGGAAGTTTACTGAATCTTCCAAAGATGGGATGACCAGGGTCTATAAGTCATTAGTGACA
AATGTCTGTAAGGAAATGTCATGTTACAGTGACTTCCCTTTCCACGAAGATTATCCTAAT
TTCATGAACCATGAAAAATTTTGGGACTATCTCCAAGAATTTGCTGAGCACTTTGACCTC
CTGAAATACATTCAGTTTAAGACCACTGTGTGCAGCATAACGAAGCGTCCAGACTTCTCC
GAAACTGGTCAGTGGGATGTTGTCACAGAGACAGAGGGCAAGCAAAATAGAGCTGTCTTT
GATGCTGTTATGGTTTGCACTGGACATTTCCTGAATCCCCATTTACCTTTGGAAGCCTTT
CCTGGAATTCATAAGTTTAAAGGTCAGATCCTGCATAGTCAAGAGTACAAGATCCCAGAA
GGCTTTCAGGGCAAACGCGTCTTGGTGATTGGTCTTGGGAACACTGGAGGAGACATTGCT
GTGGAACTCAGTCGAACGGCAGCTCAGGTACTTCTCAGTACTAGAACTGGTACCTGGGTT
CTTGGGCGCTCTTCAGATTGGGGCTATCCTTATAATATGATGGTTACAAGAAGATGCTGT
AGTTTTATTGCACAAGTTCTGCCTTCACGTTTTCTAAACTGGATTCAAGAAAGGAAGTTG
AATAAGAGATTTAATCATGAGGATTATGGATTAAGTATTACCAAAGGGAAAAAAGCAAAA
TTCATTGTGAATGATGAGCTGCCAAACTGTATCCTCTGTGGGGCAATCACTATGAAAACC
AGCGTGATTGAATTTACAGAAACCTCTGCTGTCTTTGAAGATGGGACAGTGGAAGAAAAC
ATTGATGTTGTGATCTTCACTACAGGATATACATTTTCTTTTCCATTTTTTGAAGAACCT
CTTAAAAGCCTCTGTACAAAGAAGATATTTCTATACAAGCAAGTCTTTCCCTTAAACCTA
GAGAGAGCGACATTAGCCATCATCGGCCTTATCGGCCTTAAAGGATCCATCTTATCAGGC
ACAGAGCTCCAAGCACGATGGGTCACAAGAGTATTCAAAGGACTCTGTAAGATACCTCCA
TCCCAAAAATTGATGATGGAGGCTACTGAAAAGGAACAGCTCATTAAAAGGGGAGTGTTT
AAAGACACCAGCAAAGACAAATTTGACTACATTGCCTACATGGATGATATCGCTGCCTGC
ATAGGCACAAAGCCCAGCATCCCACTTCTGTTCCTCAAGGATCCCAGACTAGCTTGGGAA
GTTTTCTTTGGACCATGTACTCCTTATCAGTACCGCCTCATGGGCCCTGGAAAATGGGAT
GGAGCCAGAAATGCCATCCTGACCCAGTGGGACAGAACATTGAAACCTTTAAAAACTCGA
ATTGTCCCTGATTCCTCCAAGCCTGCCTCCATGTCACATTATTTAAAAGCCTGGGGGGCA
CCTGTCCTACTTGCCTCTCTTCTACTTATCTGTAAATCTTCACTTTTCTTGAAATTGGTG
AGAGATAAACTACAGGACAGAATGTCCCCTTACCTAGTAAGTCTTTGGCGAGGATGA
Protein Properties
Number of Residues 558
Molecular Weight 63342.055
Theoretical pI 8.457
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>Dimethylaniline monooxygenase [N-oxide-forming] 4
MAKKVAVIGAGVSGLSSIKCCVDEDLEPTCFERSDDIGGLWKFTESSKDGMTRVYKSLVT
NVCKEMSCYSDFPFHEDYPNFMNHEKFWDYLQEFAEHFDLLKYIQFKTTVCSITKRPDFS
ETGQWDVVTETEGKQNRAVFDAVMVCTGHFLNPHLPLEAFPGIHKFKGQILHSQEYKIPE
GFQGKRVLVIGLGNTGGDIAVELSRTAAQVLLSTRTGTWVLGRSSDWGYPYNMMVTRRCC
SFIAQVLPSRFLNWIQERKLNKRFNHEDYGLSITKGKKAKFIVNDELPNCILCGAITMKT
SVIEFTETSAVFEDGTVEENIDVVIFTTGYTFSFPFFEEPLKSLCTKKIFLYKQVFPLNL
ERATLAIIGLIGLKGSILSGTELQARWVTRVFKGLCKIPPSQKLMMEATEKEQLIKRGVF
KDTSKDKFDYIAYMDDIAACIGTKPSIPLLFLKDPRLAWEVFFGPCTPYQYRLMGPGKWD
GARNAILTQWDRTLKPLKTRIVPDSSKPASMSHYLKAWGAPVLLASLLLICKSSLFLKLV
RDKLQDRMSPYLVSLWRG
GenBank ID Protein 4753758
UniProtKB/Swiss-Prot ID P31512
UniProtKB/Swiss-Prot Entry Name FMO4_HUMAN
PDB IDs Not Available
GenBank Gene ID AL031274
GeneCard ID FMO4
GenAtlas ID FMO4
HGNC ID HGNC:3772
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke G, Clee C, Cobley V, Collier RE, Corby N, Coville GJ, Davies J, Deadman R, Dunn M, Earthrowl M, Ellington AG, Errington H, Frankish A, Frankland J, French L, Garner P, Garnett J, Gay L, Ghori MR, Gibson R, Gilby LM, Gillett W, Glithero RJ, Grafham DV, Griffiths C, Griffiths-Jones S, Grocock R, Hammond S, Harrison ES, Hart E, Haugen E, Heath PD, Holmes S, Holt K, Howden PJ, Hunt AR, Hunt SE, Hunter G, Isherwood J, James R, Johnson C, Johnson D, Joy A, Kay M, Kershaw JK, Kibukawa M, Kimberley AM, King A, Knights AJ, Lad H, Laird G, Lawlor S, Leongamornlert DA, Lloyd DM, Loveland J, Lovell J, Lush MJ, Lyne R, Martin S, Mashreghi-Mohammadi M, Matthews L, Matthews NS, McLaren S, Milne S, Mistry S, Moore MJ, Nickerson T, O'Dell CN, Oliver K, Palmeiri A, Palmer SA, Parker A, Patel D, Pearce AV, Peck AI, Pelan S, Phelps K, Phillimore BJ, Plumb R, Rajan J, Raymond C, Rouse G, Saenphimmachak C, Sehra HK, Sheridan E, Shownkeen R, Sims S, Skuce CD, Smith M, Steward C, Subramanian S, Sycamore N, Tracey A, Tromans A, Van Helmond Z, Wall M, Wallis JM, White S, Whitehead SL, Wilkinson JE, Willey DL, Williams H, Wilming L, Wray PW, Wu Z, Coulson A, Vaudin M, Sulston JE, Durbin R, Hubbard T, Wooster R, Dunham I, Carter NP, McVean G, Ross MT, Harrow J, Olson MV, Beck S, Rogers J, Bentley DR, Banerjee R, Bryant SP, Burford DC, Burrill WD, Clegg SM, Dhami P, Dovey O, Faulkner LM, Gribble SM, Langford CF, Pandian RD, Porter KM, Prigmore E: The DNA sequence and biological annotation of human chromosome 1. Nature. 2006 May 18;441(7091):315-21. [PubMed:16710414 ]
  3. Furnes B, Feng J, Sommer SS, Schlenk D: Identification of novel variants of the flavin-containing monooxygenase gene family in African Americans. Drug Metab Dispos. 2003 Feb;31(2):187-93. [PubMed:12527699 ]
  4. Dolphin CT, Shephard EA, Povey S, Smith RL, Phillips IR: Cloning, primary sequence and chromosomal localization of human FMO2, a new member of the flavin-containing mono-oxygenase family. Biochem J. 1992 Oct 1;287 ( Pt 1):261-7. [PubMed:1417778 ]