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Identification
HMDB Protein ID HMDBP00377
Secondary Accession Numbers
  • 5614
Name Beta-1,4-galactosyltransferase 1
Synonyms
  1. Beta-1,4-GalTase 1
  2. Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
  3. Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
  4. Beta4Gal-T1
  5. Lactose synthase A protein
  6. N-acetyllactosamine synthase
  7. Nal synthase
  8. Processed beta-1,4-galactosyltransferase 1
  9. UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
  10. UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
  11. b4Gal-T1
Gene Name B4GALT1
Protein Type Unknown
Biological Properties
General Function Involved in transferase activity, transferring glycosyl groups
Specific Function The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Pathways
  • Congenital disorder of glycosylation CDG-IId
  • Galactose metabolism
  • Galactose Metabolism
  • Galactosemia
  • GLUT-1 deficiency syndrome
  • Glycosaminoglycan biosynthesis - keratan sulfate
  • Glycosphingolipid biosynthesis - lacto and neolacto series
  • Lactose Synthesis
  • N-Glycan biosynthesis
  • Other types of O-glycan biosynthesis
  • protein glycosylation
Reactions
Uridine diphosphategalactose + D-Glucose → Uridine 5'-diphosphate + beta-Lactose details
Uridine diphosphategalactose + N-acetyl-beta-D-glucosaminylglycopeptide → Uridine 5'-diphosphate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide details
Uridine diphosphategalactose + N-Acetyl-D-glucosamine → Uridine 5'-diphosphate + N-Acetyllactosamine details
UDP-D-galactose + Lc3Cer → UDP + Paragloboside details
UDP-D-galactose + → UDP + details
UDP-D-galactose + nLc5Cer → UDP + nLc6Cer details
UDP-D-galactose + → UDP + details
UDP-D-galactose + → UDP + details
GO Classification
Biological Process
oligosaccharide biosynthetic process
keratan sulfate biosynthetic process
acute inflammatory response
angiogenesis involved in wound healing
branching morphogenesis of a tube
development of secondary sexual characteristics
epithelial cell development
extracellular matrix organization
galactose metabolic process
lactose biosynthetic process
leukocyte migration
mammary gland development
Notch signaling pathway
penetration of zona pellucida
positive regulation of apoptotic process involved in mammary gland involution
positive regulation of epithelial cell proliferation involved in wound healing
regulation of acrosome reaction
regulation of cellular component movement
cell adhesion
post-translational protein modification
protein N-linked glycosylation via asparagine
negative regulation of cell proliferation
binding of sperm to zona pellucida
Cellular Component
Golgi cisterna membrane
desmosome
glycocalyx
Golgi trans cisterna
basolateral plasma membrane
extracellular region
brush border membrane
external side of plasma membrane
integral to membrane
Golgi membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
Molecular Function
alpha-tubulin binding
beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
lactose synthase activity
N-acetyllactosamine synthase activity
metal ion binding
protein homodimerization activity
Process
metabolic process
primary metabolic process
carbohydrate metabolic process
Cellular Location
  1. Processed beta-1
  2. 4-galactosyltransferase 1:Secreted
Gene Properties
Chromosome Location 9
Locus 9p13
SNPs B4GALT1
Gene Sequence
>1197 bp
ATGAGGCTTCGGGAGCCGCTCCTGAGCGGCAGCGCCGCGATGCCAGGCGCGTCCCTACAG
CGGGCCTGCCGCCTGCTCGTGGCCGTCTGCGCTCTGCACCTTGGCGTCACCCTCGTTTAC
TACCTGGCTGGCCGCGACCTGAGCCGCCTGCCCCAACTGGTCGGAGTCTCCACACCGCTG
CAGGGCGGCTCGAACAGTGCCGCCGCCATCGGGCAGTCCTCCGGGGAGCTCCGGACCGGA
GGGGCCCGGCCGCCGCCTCCTCTAGGCGCCTCCTCCCAGCCGCGCCCGGGTGGCGACTCC
AGCCCAGTCGTGGATTCTGGCCCTGGCCCCGCTAGCAACTTGACCTCGGTCCCAGTGCCC
CACACCACCGCACTGTCGCTGCCCGCCTGCCCTGAGGAGTCCCCGCTGCTTGTGGGCCCC
ATGCTGATTGAGTTTAACATGCCTGTGGACCTGGAGCTCGTGGCAAAGCAGAACCCAAAT
GTGAAGATGGGCGGCCGCTATGCCCCCAGGGACTGCGTCTCTCCTCACAAGGTGGCCATC
ATCATTCCATTCCGCAACCGGCAGGAGCACCTCAAGTACTGGCTATATTATTTGCACCCA
GTCCTGCAGCGCCAGCAGCTGGACTATGGCATCTATGTTATCAACCAGGCGGGAGACACT
ATATTCAATCGTGCTAAGCTCCTCAATGTTGGCTTTCAAGAAGCCTTGAAGGACTATGAC
TACACCTGCTTTGTGTTTAGCGACGTGGACCTCATTCCAATGAATGACCATAATGCGTAC
AGGTGTTTTTCACAGCCACGGCACATTTCCGTTGCAATGGATAAGTTTGGATTCAGCCTA
CCTTATGTTCAGTATTTTGGAGGTGTCTCTGCTCTAAGTAAACAACAGTTTCTAACCATC
AATGGATTTCCTAATAATTATTGGGGCTGGGGAGGAGAAGATGATGACATTTTTAACAGA
TTAGTTTTTAGAGGCATGTCTATATCTCGCCCAAATGCTGTGGTCGGGAGGTGTCGCATG
ATCCGCCACTCAAGAGACAAGAAAAATGAACCCAATCCTCAGAGGTTTGACCGAATTGCA
CACACAAAGGAGACAATGCTCTCTGATGGTTTGAACTCACTCACCTACCAGGTGCTGGAT
GTACAGAGATACCCATTGTATACCCAAATCACAGTGGACATCGGGACACCGAGCTAG
Protein Properties
Number of Residues 398
Molecular Weight 43919.895
Theoretical pI 8.642
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>Beta-1,4-galactosyltransferase 1
MRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLSRLPQLVGVSTPL
QGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVP
HTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKMGGRYAPRDCVSPHKVAI
IIPFRNRQEHLKYWLYYLHPVLQRQQLDYGIYVINQAGDTIFNRAKLLNVGFQEALKDYD
YTCFVFSDVDLIPMNDHNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLTI
NGFPNNYWGWGGEDDDIFNRLVFRGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIA
HTKETMLSDGLNSLTYQVLDVQRYPLYTQITVDIGTPS
GenBank ID Protein 189053491
UniProtKB/Swiss-Prot ID P15291
UniProtKB/Swiss-Prot Entry Name B4GT1_HUMAN
PDB IDs
GenBank Gene ID AK312797
GeneCard ID B4GALT1
GenAtlas ID B4GALT1
HGNC ID HGNC:924
References
General References
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  3. Amado M, Almeida R, Schwientek T, Clausen H: Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1999 Dec 6;1473(1):35-53. [PubMed:10580128 ]
  4. Masri KA, Appert HE, Fukuda MN: Identification of the full-length coding sequence for human galactosyltransferase (beta-N-acetylglucosaminide: beta 1,4-galactosyltransferase). Biochem Biophys Res Commun. 1988 Dec 15;157(2):657-63. [PubMed:3144273 ]
  5. Watzele G, Berger EG: Near identity of HeLa cell galactosyltransferase with the human placental enzyme. Nucleic Acids Res. 1990 Dec 11;18(23):7174. [PubMed:2124683 ]
  6. Mengle-Gaw L, McCoy-Haman MF, Tiemeier DC: Genomic structure and expression of human beta-1,4-galactosyltransferase. Biochem Biophys Res Commun. 1991 May 15;176(3):1269-76. [PubMed:1903938 ]
  7. Uejima T, Uemura M, Nozawa S, Narimatsu H: Complementary DNA cloning for galactosyltransferase associated with tumor and determination of antigenic epitopes recognized by specific monoclonal antibodies. Cancer Res. 1992 Nov 15;52(22):6158-63. [PubMed:1384956 ]
  8. Kudo T, Narimatsu H: The beta 1,4-galactosyltransferase gene is post-transcriptionally regulated during differentiation of mouse F9 teratocarcinoma cells. Glycobiology. 1995 Jun;5(4):397-403. [PubMed:7579794 ]
  9. Chatterjee SK, Mukerjee S, Tripathi PK: Analysis of the sequences of human beta-1,4-galactosyltransferase cDNA clones. Int J Biochem Cell Biol. 1995 Mar;27(3):329-36. [PubMed:7540104 ]
  10. Appert HE, Rutherford TJ, Tarr GE, Wiest JS, Thomford NR, McCorquodale DJ: Isolation of a cDNA coding for human galactosyltransferase. Biochem Biophys Res Commun. 1986 Aug 29;139(1):163-8. [PubMed:3094506 ]
  11. Appert HE, Rutherford TJ, Tarr GE, Thomford NR, McCorquodale DJ: Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence. Biochem Biophys Res Commun. 1986 Jul 16;138(1):224-9. [PubMed:3091013 ]
  12. Aoki D, Appert HE, Johnson D, Wong SS, Fukuda MN: Analysis of the substrate binding sites of human galactosyltransferase by protein engineering. EMBO J. 1990 Oct;9(10):3171-8. [PubMed:2120039 ]
  13. Lopez LC, Youakim A, Evans SC, Shur BD: Evidence for a molecular distinction between Golgi and cell surface forms of beta 1,4-galactosyltransferase. J Biol Chem. 1991 Aug 25;266(24):15984-91. [PubMed:1714903 ]
  14. Yamaguchi N, Fukuda MN: Golgi retention mechanism of beta-1,4-galactosyltransferase. Membrane-spanning domain-dependent homodimerization and association with alpha- and beta-tubulins. J Biol Chem. 1995 May 19;270(20):12170-6. [PubMed:7744867 ]