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Identification
HMDB Protein ID HMDBP01096
Secondary Accession Numbers
  • 6385
Name UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Synonyms
  1. G1PT
  2. GPT
  3. GlcNAc-1-P transferase
  4. N-acetylglucosamine-1-phosphate transferase
Gene Name DPAGT1
Protein Type Enzyme
Biological Properties
General Function Involved in phospho-N-acetylmuramoyl-pentapeptide-transferase activity
Specific Function Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.
Pathways
  • N-Glycan biosynthesis
  • protein glycosylation
Reactions
Uridine diphosphate-N-acetylglucosamine + Dolichol-20 → Uridine 5'-monophosphate + N-acetyl-D-glucosaminyl-diphosphodolichol details
Uridine diphosphate-N-acetylglucosamine + Dolichol-20 → Uridine 5'-monophosphate + N-Acetyl-D-glucosaminyldiphosphodolichol details
GO Classification
Biological Process
dolichol biosynthetic process
dolichol-linked oligosaccharide biosynthetic process
post-translational protein modification
protein N-linked glycosylation via asparagine
protein oligomerization
UDP-N-acetylglucosamine metabolic process
Cellular Component
integral to endoplasmic reticulum membrane
Component
cell part
membrane part
intrinsic to membrane
integral to membrane
Function
catalytic activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, for other substituted phosphate groups
phospho-n-acetylmuramoyl-pentapeptide-transferase activity
Molecular Function
phospho-N-acetylmuramoyl-pentapeptide-transferase activity
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity
transferase activity, transferring glycosyl groups
Cellular Location
  1. Endoplasmic reticulum membrane
  2. Multi-pass membrane protein
Gene Properties
Chromosome Location 11
Locus 11q23.3
SNPs DPAGT1
Gene Sequence
>1227 bp
ATGTGGGCCTTCTCGGAATTGCCCATGCCGCTGCTGATCAATTTGATCGTCTCGCTGCTG
GGATTTGTGGCCACAGTCACCCTCATCCCGGCCTTCCGGGGCCACTTCATTGCTGCGCGC
CTCTGTGGTCAGGACCTCAACAAAACCAGCCGACAGCAGATCCCAGAATCCCAGGGAGTG
ATCAGCGGTGCTGTTTTCCTTATCATCCTCTTCTGCTTCATCCCTTTCCCCTTCCTGAAC
TGCTTTGTGAAGGAGCAGTGTAAGGCATTCCCCCACCATGAATTTGTGGCCCTGATAGGT
GCCCTCCTTGCCATCTGCTGCATGATCTTCCTGGGCTTTGCGGATGATGTACTGAATCTG
CGCTGGCGCCATAAGCTGCTGCTACATACAGCTGCCTCACTACCTCTCCTCATGGTATAT
TTCACCAACTTTGGCAACACGACCATTGTGGTGCCCAAGCCTTTCCGCCCGATACTTGGC
CTGCATCTGGACTTGGGAATCCTGTACTATGTCTACATGGGGCTGCTGGCAGTGTTCTGT
ACCAATGCCATCAATATCCTAGCAGGAATTAACGGCCTAGAGGCTGGCCAGTCACTAGTC
ATTTCTGCTTCCATCATTGTCTTCAACCTGGTAGAGTTGGAAGGTGATTGTCGGGATGAT
CATGTCTTTTCCCTCTACTTCATGATACCCTTTTTTTTCACCACTTTGGGATTGCTCTAC
CACAACTGGTACCCATCACGGGTGTTTGTGGGAGATACCTTCTGTTACTTTGCTGGCATG
ACCTTTGCCGTGGTGGGCATCTTGGGACACTTCAGCAAGACCATGCTACTATTCTTCATG
CCCCAGGTGTTCAACTTCCTCTACTCACTGCCTCAGCTCCTGCATATCATCCCCTGCCCT
CGCCACCGCATACCCAGACTCAATATCAAGACAGGCAAACTGGAGATGAGCTATTCCAAG
TTCAAGACCAAGAGCCTCTCTTTCTTGGGCACCTTTATTTTAAAGGTGGCAGAGAGCCTC
CAGCTGGTGACAGTACACCAGAGTGAGACTGAAGATGGTGAATTCACTGAATGTAACAAC
ATGACCCTCATCAACTTGCTACTTAAAGTCCTTGGGCCCATACATGAGAGAAACCTCACA
TTGCTCCTGCTGCTGCTGCAGATCCTGGGCAGTGCCATCACCTTCTCCATTCGATATCAG
CTCGTTCGACTCTTCTATGATGTCTGA
Protein Properties
Number of Residues 408
Molecular Weight 46089.485
Theoretical pI 7.997
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence
>UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
MWAFSELPMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGV
ISGAVFLIILFCFIPFPFLNCFVKEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNL
RWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRPILGLHLDLGILYYVYMGLLAVFC
TNAINILAGINGLEAGQSLVISASIIVFNLVELEGDCRDDHVFSLYFMIPFFFTTLGLLY
HNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCP
RHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSETEDGEFTECNN
MTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQLVRLFYDV
GenBank ID Protein 12002052
UniProtKB/Swiss-Prot ID Q9H3H5
UniProtKB/Swiss-Prot Entry Name GPT_HUMAN
PDB IDs Not Available
GenBank Gene ID AF070443
GeneCard ID DPAGT1
GenAtlas ID DPAGT1
HGNC ID HGNC:2995
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Sjoblom T, Jones S, Wood LD, Parsons DW, Lin J, Barber TD, Mandelker D, Leary RJ, Ptak J, Silliman N, Szabo S, Buckhaults P, Farrell C, Meeh P, Markowitz SD, Willis J, Dawson D, Willson JK, Gazdar AF, Hartigan J, Wu L, Liu C, Parmigiani G, Park BH, Bachman KE, Papadopoulos N, Vogelstein B, Kinzler KW, Velculescu VE: The consensus coding sequences of human breast and colorectal cancers. Science. 2006 Oct 13;314(5797):268-74. Epub 2006 Sep 7. [PubMed:16959974 ]
  3. Eckert V, Blank M, Mazhari-Tabrizi R, Mumberg D, Funk M, Schwarz RT: Cloning and functional expression of the human GlcNAc-1-P transferase, the enzyme for the committed step of the dolichol cycle, by heterologous complementation in Saccharomyces cerevisiae. Glycobiology. 1998 Jan;8(1):77-85. [PubMed:9451016 ]
  4. Wu X, Rush JS, Karaoglu D, Krasnewich D, Lubinsky MS, Waechter CJ, Gilmore R, Freeze HH: Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of Glycosylation Type Ij. Hum Mutat. 2003 Aug;22(2):144-50. [PubMed:12872255 ]