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Identification
HMDB Protein ID HMDBP01614
Secondary Accession Numbers
  • 6937
Name Neutrophil cytosol factor 2
Synonyms
  1. 67 kDa neutrophil oxidase factor
  2. NADPH oxidase activator 2
  3. NCF-2
  4. Neutrophil NADPH oxidase factor 2
  5. p67-phox
Gene Name NCF2
Protein Type Enzyme
Biological Properties
General Function Involved in electron carrier activity
Specific Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production)
Pathways Not Available
Reactions Not Available
GO Classification
Function
binding
Cellular Location
  1. Cytoplasm
Gene Properties
Chromosome Location Chromosome:1
Locus 1q25
SNPs NCF2
Gene Sequence
>1581 bp
ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAG
GACTGGAAGGGAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGC
TTCAACATTGGCTGCATGTACACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTT
ACCAGAAGCATTAACCGAGACAAGCACTTGGCAGTGGCTTACTTCCAACGAGGGATGCTC
TACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACCTTAAAGAAGCCTTGATTCAG
CTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAAGCTGTTTGCC
TGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAATGGAAAAAAGCT
GAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCAAAATCGAC
AAGGCGATGGAGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGC
AGGCTGTTTCGACCAAATGAGAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGC
AAGGCGACGGTCGTGGCATCTGTGGTGGATCAAGACAGTTTCTCTGGGTTTGCCCCTCTG
CAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCCCAGAGATCTTCAGGGCTCTG
GAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAAGAAGAGCTCCAG
GTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCACGGTC
ATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGG
ATCCACCCTCAGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCT
CCTAGTTCCAAAGCCCCTGGAAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAG
CCTAAGGAAGTGAAGCTCAGTGTTCCCATGCCCTACACACTCAAGGTGCACTACAAGTAC
ACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCCAGGTCCGGGACATGGTGTCT
AAGAAACTGGAGCTCCGGCTGGAACACACTAAGCTGAGCTATCGGCCTCGGGACAGCAAT
GAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAAACTAC
TGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAG
GAAAGTGAAAAAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGC
CAAGTGGAGGCACTCTTCAGTTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAA
GGGGATATAATCCTGGTGTTATCAAAGGTGAATGAAGAATGGCTGGAAGGGGAGTGCAAA
GGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACTGCGCAACTACAGATTTGGAA
AGCACTCGGAGAGAAGTCTAG
Protein Properties
Number of Residues 526
Molecular Weight 59761.1
Theoretical pI 6.02
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>Neutrophil cytosol factor 2
MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAF
TRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFA
CEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVG
KLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRAL
EGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELR
IHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKY
TVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNY
CLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQE
GDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV
GenBank ID Protein 22023953
UniProtKB/Swiss-Prot ID P19878
UniProtKB/Swiss-Prot Entry Name NCF2_HUMAN
PDB IDs
GenBank Gene ID AF527950
GeneCard ID NCF2
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
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  4. Leto TL, Lomax KJ, Volpp BD, Nunoi H, Sechler JM, Nauseef WM, Clark RA, Gallin JI, Malech HL: Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src. Science. 1990 May 11;248(4956):727-30. [PubMed:1692159 ]
  5. Kenney RT, Malech HL, Epstein ND, Roberts RL, Leto TL: Characterization of the p67phox gene: genomic organization and restriction fragment length polymorphism analysis for prenatal diagnosis in chronic granulomatous disease. Blood. 1993 Dec 15;82(12):3739-44. [PubMed:7903171 ]
  6. Yoshida L, Nishida S, Shimoyama T, Kawahara T, Rokutan K, Tsunawaki S: Expression of a p67(phox) homolog in Caco-2 cells giving O(2)(-)-reconstituting ability to cytochrome b(558) together with recombinant p47(phox). Biochem Biophys Res Commun. 2002 Sep 6;296(5):1322-8. [PubMed:12207919 ]
  7. McAdara Berkowitz JK, Catz SD, Johnson JL, Ruedi JM, Thon V, Babior BM: JFC1, a novel tandem C2 domain-containing protein associated with the leukocyte NADPH oxidase. J Biol Chem. 2001 Jun 1;276(22):18855-62. Epub 2001 Mar 13. [PubMed:11278853 ]
  8. Takeya R, Ueno N, Kami K, Taura M, Kohjima M, Izaki T, Nunoi H, Sumimoto H: Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases. J Biol Chem. 2003 Jul 4;278(27):25234-46. Epub 2003 Apr 25. [PubMed:12716910 ]
  9. Lapouge K, Smith SJ, Walker PA, Gamblin SJ, Smerdon SJ, Rittinger K: Structure of the TPR domain of p67phox in complex with Rac.GTP. Mol Cell. 2000 Oct;6(4):899-907. [PubMed:11090627 ]
  10. de Boer M, Hilarius-Stokman PM, Hossle JP, Verhoeven AJ, Graf N, Kenney RT, Seger R, Roos D: Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers. Blood. 1994 Jan 15;83(2):531-6. [PubMed:8286749 ]
  11. Bonizzato A, Russo MP, Donini M, Dusi S: Identification of a double mutation (D160V-K161E) in the p67phox gene of a chronic granulomatous disease patient. Biochem Biophys Res Commun. 1997 Feb 24;231(3):861-3. [PubMed:9070911 ]
  12. Patino PJ, Rae J, Noack D, Erickson R, Ding J, de Olarte DG, Curnutte JT: Molecular characterization of autosomal recessive chronic granulomatous disease caused by a defect of the nicotinamide adenine dinucleotide phosphate (reduced form) oxidase component p67-phox. Blood. 1999 Oct 1;94(7):2505-14. [PubMed:10498624 ]
  13. Noack D, Rae J, Cross AR, Munoz J, Salmen S, Mendoza JA, Rossi N, Curnutte JT, Heyworth PG: Autosomal recessive chronic granulomatous disease caused by novel mutations in NCF-2, the gene encoding the p67-phox component of phagocyte NADPH oxidase. Hum Genet. 1999 Nov;105(5):460-7. [PubMed:10598813 ]