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Identification
HMDB Protein ID HMDBP01887
Secondary Accession Numbers
  • 7286
Name Protein S100-A8
Synonyms
  1. CFAG
  2. Calgranulin-A
  3. Calprotectin L1L subunit
  4. Cystic fibrosis antigen
  5. Leukocyte L1 complex light chain
  6. MRP-8
  7. Migration inhibitory factor-related protein 8
  8. S100 calcium-binding protein A8
  9. Urinary stone protein band A
  10. p8
Gene Name S100A8
Protein Type Unknown
Biological Properties
General Function Involved in calcium ion binding
Specific Function Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro- inflammatory mediator in acute and chronic inflammation and up- regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions
Pathways Not Available
Reactions Not Available
GO Classification
Function
ion binding
cation binding
metal ion binding
binding
calcium ion binding
Cellular Location
  1. Cell membrane
  2. Cytoplasm
  3. Cytoplasm
  4. Peripheral membrane protein
  5. Secreted
  6. cytoskeleton
Gene Properties
Chromosome Location Chromosome:1
Locus 1q21
SNPs S100A8
Gene Sequence
>282 bp
ATGTTGACCGAGCTGGAGAAAGCCTTGAACTCTATCATCGACGTCTACCACAAGTACTCC
CTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGAAGAAATTGCTAGAGACC
GAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGGTTCAAAGAGTTGGATATC
AACACTGATGGTGCAGTTAACTTCCAGGAGTTCCTCATTCTGGTGATAAAGATGGGCGTG
GCAGCCCACAAAAAAAGCCATGAAGAAAGCCACAAAGAGTAG
Protein Properties
Number of Residues 93
Molecular Weight 10834.4
Theoretical pI 7.07
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>Protein S100-A8
MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDI
NTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE
GenBank ID Protein 158256092
UniProtKB/Swiss-Prot ID P05109
UniProtKB/Swiss-Prot Entry Name S10A8_HUMAN
PDB IDs
GenBank Gene ID AK291328
GeneCard ID S100A8
GenAtlas ID S100A8
HGNC ID HGNC:10498
References
General References
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  3. Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke G, Clee C, Cobley V, Collier RE, Corby N, Coville GJ, Davies J, Deadman R, Dunn M, Earthrowl M, Ellington AG, Errington H, Frankish A, Frankland J, French L, Garner P, Garnett J, Gay L, Ghori MR, Gibson R, Gilby LM, Gillett W, Glithero RJ, Grafham DV, Griffiths C, Griffiths-Jones S, Grocock R, Hammond S, Harrison ES, Hart E, Haugen E, Heath PD, Holmes S, Holt K, Howden PJ, Hunt AR, Hunt SE, Hunter G, Isherwood J, James R, Johnson C, Johnson D, Joy A, Kay M, Kershaw JK, Kibukawa M, Kimberley AM, King A, Knights AJ, Lad H, Laird G, Lawlor S, Leongamornlert DA, Lloyd DM, Loveland J, Lovell J, Lush MJ, Lyne R, Martin S, Mashreghi-Mohammadi M, Matthews L, Matthews NS, McLaren S, Milne S, Mistry S, Moore MJ, Nickerson T, O'Dell CN, Oliver K, Palmeiri A, Palmer SA, Parker A, Patel D, Pearce AV, Peck AI, Pelan S, Phelps K, Phillimore BJ, Plumb R, Rajan J, Raymond C, Rouse G, Saenphimmachak C, Sehra HK, Sheridan E, Shownkeen R, Sims S, Skuce CD, Smith M, Steward C, Subramanian S, Sycamore N, Tracey A, Tromans A, Van Helmond Z, Wall M, Wallis JM, White S, Whitehead SL, Wilkinson JE, Willey DL, Williams H, Wilming L, Wray PW, Wu Z, Coulson A, Vaudin M, Sulston JE, Durbin R, Hubbard T, Wooster R, Dunham I, Carter NP, McVean G, Ross MT, Harrow J, Olson MV, Beck S, Rogers J, Bentley DR, Banerjee R, Bryant SP, Burford DC, Burrill WD, Clegg SM, Dhami P, Dovey O, Faulkner LM, Gribble SM, Langford CF, Pandian RD, Porter KM, Prigmore E: The DNA sequence and biological annotation of human chromosome 1. Nature. 2006 May 18;441(7091):315-21. [PubMed:16710414 ]
  4. Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J: Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes. Electrophoresis. 1992 Dec;13(12):960-9. [PubMed:1286667 ]
  5. Dorin JR, Novak M, Hill RE, Brock DJ, Secher DS, van Heyningen V: A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene. Nature. 1987 Apr 9-15;326(6113):614-7. [PubMed:3561500 ]
  6. Odink K, Cerletti N, Bruggen J, Clerc RG, Tarcsay L, Zwadlo G, Gerhards G, Schlegel R, Sorg C: Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature. 1987 Nov 5-11;330(6143):80-2. [PubMed:3313057 ]
  7. Lagasse E, Clerc RG: Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation. Mol Cell Biol. 1988 Jun;8(6):2402-10. [PubMed:3405210 ]
  8. Schafer T, Sachse GE, Gassen HG: The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells. Biol Chem Hoppe Seyler. 1991 Jan;372(1):1-4. [PubMed:2039599 ]
  9. Marti T, Erttmann KD, Gallin MY: Host-parasite interaction in human onchocerciasis: identification and sequence analysis of a novel human calgranulin. Biochem Biophys Res Commun. 1996 Apr 16;221(2):454-8. [PubMed:8619876 ]
  10. Miyasaki KT, Bodeau AL, Murthy AR, Lehrer RI: In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena. J Dent Res. 1993 Feb;72(2):517-23. [PubMed:8423249 ]
  11. Lemarchand P, Vaglio M, Mauel J, Markert M: Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation. J Biol Chem. 1992 Sep 25;267(27):19379-82. [PubMed:1326551 ]
  12. Umekawa T, Kurita T: Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone. Biochem Mol Biol Int. 1994 Sep;34(2):309-13. [PubMed:7849642 ]
  13. Nakai M, Ishikawa M, Hamada Y, Sugano S: Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8. Biol Chem Hoppe Seyler. 1994 Nov;375(11):789-92. [PubMed:7695842 ]
  14. Rammes A, Roth J, Goebeler M, Klempt M, Hartmann M, Sorg C: Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway. J Biol Chem. 1997 Apr 4;272(14):9496-502. [PubMed:9083090 ]
  15. Vogl T, Ludwig S, Goebeler M, Strey A, Thorey IS, Reichelt R, Foell D, Gerke V, Manitz MP, Nacken W, Werner S, Sorg C, Roth J: MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes. Blood. 2004 Dec 15;104(13):4260-8. Epub 2004 Aug 26. [PubMed:15331440 ]
  16. Viemann D, Strey A, Janning A, Jurk K, Klimmek K, Vogl T, Hirono K, Ichida F, Foell D, Kehrel B, Gerke V, Sorg C, Roth J: Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells. Blood. 2005 Apr 1;105(7):2955-62. Epub 2004 Dec 14. [PubMed:15598812 ]
  17. Nakatani Y, Yamazaki M, Chazin WJ, Yui S: Regulation of S100A8/A9 (calprotectin) binding to tumor cells by zinc ion and its implication for apoptosis-inducing activity. Mediators Inflamm. 2005 Oct 24;2005(5):280-92. [PubMed:16258195 ]
  18. Bode G, Luken A, Kerkhoff C, Roth J, Ludwig S, Nacken W: Interaction between S100A8/A9 and annexin A6 is involved in the calcium-induced cell surface exposition of S100A8/A9. J Biol Chem. 2008 Nov 14;283(46):31776-84. doi: 10.1074/jbc.M803908200. Epub 2008 Sep 11. [PubMed:18786929 ]
  19. Sroussi HY, Kohler GA, Agabian N, Villines D, Palefsky JM: Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation. FEMS Immunol Med Microbiol. 2009 Jan;55(1):55-61. doi: 10.1111/j.1574-695X.2008.00498.x. Epub 2008 Dec 11. [PubMed:19087201 ]
  20. Champaiboon C, Sappington KJ, Guenther BD, Ross KF, Herzberg MC: Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion. J Biol Chem. 2009 Mar 13;284(11):7078-90. doi: 10.1074/jbc.M806605200. Epub 2009 Jan 3. [PubMed:19122197 ]
  21. Ishikawa K, Nakagawa A, Tanaka I, Suzuki M, Nishihira J: The structure of human MRP8, a member of the S100 calcium-binding protein family, by MAD phasing at 1.9 A resolution. Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):559-66. [PubMed:10771424 ]
  22. Korndorfer IP, Brueckner F, Skerra A: The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins. J Mol Biol. 2007 Jul 27;370(5):887-98. Epub 2007 May 3. [PubMed:17553524 ]