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Identification
HMDB Protein ID HMDBP08459
Secondary Accession Numbers
  • 14171
Name Importin subunit alpha-4
Synonyms
  1. Importin alpha Q1
  2. Karyopherin subunit alpha-4
  3. Qip1
Gene Name KPNA4
Protein Type Unknown
Biological Properties
General Function Involved in binding
Specific Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a nonclassical NLS
Pathways Not Available
Reactions Not Available
GO Classification
Component
cell part
intracellular part
cytoplasm
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
nucleus
nuclear pore
organelle part
intracellular organelle part
nuclear part
Function
binding
protein transporter activity
transporter activity
substrate-specific transporter activity
Process
establishment of localization
transport
protein transport
protein import into nucleus
intracellular protein transport
protein targeting
Cellular Location
  1. Nucleus
  2. Cytoplasm
Gene Properties
Chromosome Location Chromosome:3
Locus 3q25.33
SNPs KPNA4
Gene Sequence
>1566 bp
ATGGCGGACAACGAGAAACTGGACAACCAACGGCTCAAGAATTTCAAGAACAAAGGCCGC
GACTTGGAGACTATGAGAAGACAACGAAATGAAGTTGTAGTTGAATTAAGGAAGAATAAA
AGAGATGAACATCTCTTAAAGAGAAGGAATGTACCACATGAAGATATCTGTGAAGACTCT
GATATAGATGGTGATTATAGAGTGCAAAATACCTCTCTAGAAGCTATTGTTCAAAATGCT
TCAAGTGATAACCAAGGAATTCAATTAAGTGCAGTTCAAGCTGCTAGGAAGCTTTTGTCC
AGTGATCGAAATCCACCAATTGATGACTTAATAAAATCTGGAATATTGCCCATTTTAGTC
CATTGTCTTGAAAGAGATGACAATCCTTCTTTACAGTTTGAAGCTGCATGGGCTTTGACA
AACATTGCATCTGGAACTTCTGAACAAACTCAAGCAGTAGTTCAGTCCAATGCTGTGCCA
CTTTTCCTGAGGCTTCTCCATTCACCCCATCAGAATGTCTGTGAGCAAGCAGTGTGGGCA
TTGGGAAATATCATAGGTGATGGGCCCCAGTGTAGAGATTATGTCATAAGTCTTGGAGTT
GTGAAACCTTTACTTTCCTTCATAAGTCCATCTATTCCTATAACATTCTTAAGAAATGTT
ACTTGGGTTATGGTCAACTTATGTCGCCACAAAGACCCACCACCACCAATGGAAACCATT
CAGGAGATTCTTCCAGCCCTTTGTGTTTTAATTCATCACACAGATGTAAATATACTGGTA
GACACAGTCTGGGCCCTCTCTTACCTTACTGATGCTGGCAATGAACAAATACAGATGGTA
ATAGACTCTGGAATAGTTCCTCATTTGGTTCCTCTGCTCAGCCACCAGGAAGTTAAAGTT
CAGACTGCTGCACTTAGAGCTGTGGGCAACATTGTTACTGGAACTGATGAGCAAACACAA
GTAGTTTTGAACTGTGATGCTCTTTCACACTTCCCAGCACTCCTGACACATCCCAAAGAG
AAAATTAATAAAGAAGCAGTGTGGTTCCTCTCCAACATCACTGCAGGAAATCAGCAGCAG
GTACAGGCAGTAATTGATGCCAATCTTGTACCAATGATAATACACCTTTTGGATAAGGGG
GATTTTGGCACTCAAAAAGAAGCTGCTTGGGCCATAAGTAACTTAACAATTAGTGGAAGG
AAAGATCAAGTGGCTTACCTTATCCAACAAAATGTTATCCCACCTTTTTGCAACTTGCTG
ACTGTAAAAGATGCACAAGTTGTGCAAGTAGTACTCGATGGACTAAGTAATATATTAAAA
ATGGCTGAAGATGAGGCAGAAACCATAGGCAATCTTATAGAAGAATGTGGAGGGCTGGAG
AAAATTGAACAACTTCAAAATCATGAAAATGAAGACATCTACAAATTGGCCTATGAGATC
ATTGATCAGTTCTTCTCTTCAGATGATATTGATGAAGACCCTAGCCTTGTTCCAGAGGCA
ATTCAAGGCGGAACATTTGGTTTCAATTCATCTGCCAATGTACCAACAGAAGGGTTCCAG
TTTTAG
Protein Properties
Number of Residues 521
Molecular Weight 57886.3
Theoretical pI 4.56
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>Importin subunit alpha-4
MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDS
DIDGDYRVQNTSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPILV
HCLERDDNPSLQFEAAWALTNIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWA
LGNIIGDGPQCRDYVISLGVVKPLLSFISPSIPITFLRNVTWVMVNLCRHKDPPPPMETI
QEILPALCVLIHHTDVNILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKV
QTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFLSNITAGNQQQ
VQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGRKDQVAYLIQQNVIPPFCNLL
TVKDAQVVQVVLDGLSNILKMAEDEAETIGNLIEECGGLEKIEQLQNHENEDIYKLAYEI
IDQFFSSDDIDEDPSLVPEAIQGGTFGFNSSANVPTEGFQF
GenBank ID Protein 1944125
UniProtKB/Swiss-Prot ID O00629
UniProtKB/Swiss-Prot Entry Name IMA4_HUMAN
PDB IDs Not Available
GenBank Gene ID AB002533
GeneCard ID KPNA4
GenAtlas ID KPNA4
HGNC ID HGNC:6397
References
General References
  1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [PubMed:14702039 ]
  2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  3. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
  4. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [PubMed:19690332 ]
  5. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. [PubMed:17081983 ]
  6. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [PubMed:19413330 ]
  7. Lischka P, Sorg G, Kann M, Winkler M, Stamminger T: A nonconventional nuclear localization signal within the UL84 protein of human cytomegalovirus mediates nuclear import via the importin alpha/beta pathway. J Virol. 2003 Mar;77(6):3734-48. [PubMed:12610148 ]
  8. Kohler M, Ansieau S, Prehn S, Leutz A, Haller H, Hartmann E: Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family. FEBS Lett. 1997 Nov 3;417(1):104-8. [PubMed:9395085 ]
  9. Seki T, Tada S, Katada T, Enomoto T: Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL. Biochem Biophys Res Commun. 1997 May 8;234(1):48-53. [PubMed:9168958 ]