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Identification
HMDB Protein ID HMDBP08515
Secondary Accession Numbers
  • 14227
Name Rab5 GDP/GTP exchange factor
Synonyms
  1. RAP1
  2. Rabaptin-5-associated exchange factor for Rab5
  3. Rabex-5
Gene Name RABGEF1
Protein Type Unknown
Biological Properties
General Function Involved in DNA binding
Specific Function Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase
Pathways Not Available
Reactions Not Available
GO Classification
Function
ion binding
cation binding
metal ion binding
binding
transition metal ion binding
zinc ion binding
nucleic acid binding
dna binding
Cellular Location
  1. Cytoplasm
  2. Early endosome
  3. Recycling endosome
Gene Properties
Chromosome Location Chromosome:7
Locus 7q11.21
SNPs RABGEF1
Gene Sequence
>2127 bp
ATGGTGGTAGTCACGGGGCGGGAGCCAGACAGCCGTCGTCAGGACGGTGCCATGTCCAGC
TCTGACGCCGAAGACGACTTTCTGGAGCCGGCCACGCCGACGGCCACGCAGGCGGGGCAC
GCGCTGCCCCTGCTGCCACAGGAGAGATGTGCTGAATTTCCTGCGCTCAGGGGACCTCCC
ACCCAGGGAGCGTGTTCGAGCTGTGTACAAAGAGGCCCAGTACTATGCCATCGGGCCCCT
CCTGGAGCAGCTGGAGAACATGCAGCCACTGAAGGGCGAGAAGGTGCGCCAAGCGTTTCT
GGGACTCATGCCCTATTACAAAGACCACTTGGAGCGGATTGTGGAGATCGCCCGGCTGCG
TGCGGTCCAGCGGAAGGCCCGCTTTGCCAAGCTCAAGTGGTTAGCAGGAAGAAGATGAGC
CTTAAGTCTGAACGCCGAGGAATTCATGTGGATCAATCGGATCTCCTGTGCAAGAAAGGA
TGTGGTTACTACGGCAACCCTGCCTGGCAGGGTTTCTGCTCCAAGTGCTGGAGGGAAGAG
TACCACAAAGCCAGGCAGAAGCAGATTCAGGAGGACTGGGAGCTGGCGGAGCGGGTCTTG
CTCTGTTGCCCTGGCTGGAGTGCAATGGTGCAATTTCAACTTACTGCAACCTCTGCTTCC
TGGGCTCAAGTGATCCTCCTGCTTCAGCCTCCCAAGTGGCTGGGACTACAGAAACTCCAG
CGGGAGGAAGAAGAGGCCTTTGCCAGCAGTCAGAGCAGCCAAGGGGCCCAATCCCTCACA
TTCTCCAAGTTTGAAGAAAAGAAAACCAACGAGAAGACCCGCAAGGTTACCACAGTGAAG
AAATTCTTCAGTGCATCTTCCAGGGTCGGATCAAAGAAGGAAATTCAGGAAGCAAAAGCT
CCCAGTCCTTCCATAAACCGGCAAACCAGCATTGAAACGGATAGAGTGTCTAAGGAGTTC
ATAGAATTTCTCAAGACCTTCCACAAGACAGGCCAAGAAATCTATAAACAGACCAAGCTG
TTTTTGGAAGGAATGCATTACAAAAGGGATCTAAGCATTGAAGAACAGTCAGAGTGTGCT
CAGGATTTCTACCACAATGTGGCCGAAAGGATGCAAACTCGTGGGAAAGAGAGGAGATTT
CACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGCGATCCACCTGCCTCAGCC
TCCCAAAGTGCTGGGAATACAGGAGTAGAGCCACCGCACCCAGCTGTGCCTCCAGAAAGA
GTCGAGAAGATAATGGATCAGATTGAAAAGTACATCATGACTCGTCTCTATAAATATGTA
TTCTGTCCAGAAACTACTGATGATGAGAAGAAAGATCTTGCCATTCAAAAGAGAATCAGA
GCCCTGCGCTGGGTTACGCCTCAGATGCTGTGTGTCCCTGTTAATGACGACATCCCAGAA
GTATCTGATATGGTGGTGAAGGCGATCACAGATATCATTGAAATGGATTCCAAGCGTGTG
CCTCGAGACAAGCTGGCCTGCATCACCAAGTGCAGCAAGCACATCTTCAATGCCATCAAG
ATCACCAAGAATGAGCCGGCGTCAGCGGATGACTTCCTCCCCACCCTCATCTACATTGTT
TTGAAGGGCAACCCCCCACGCCTTCAGTCTAATATCCAGTATATCACGCGCTTCTGCAAT
CCAAGCCGACTGATGACTGGAGAGGATGGCTACTATTTCACCAATCTGTGCTGTGCTGTG
GCTTTCATTGAGAAGCTAGACGCCCAGTCTTTGAATCTAAGTCAGGAGGATTTTGATCGC
TACATGTCTGGCCAGACCTCTCCCAGGAAGCAAGAAGCTGAGAGTTGGTCTCCTGATGCT
TGCTTAGGCGTCAAGCAAATGTATAAGAACTTGGATCTCTTGTCTCAGTTGAATGAACGA
CAAGAAAGGATCATGAATGAAGCCAAGAAACTGGAAAAAGACCTCATAGATTGGACAGAT
GGAATTGCAAGAGAAGTTCAAGACATCGTTGAGAAATACCCACTGGAAATTAAGCCTCCG
AATCAACCGTTAGCAGCTATTGACTCTGAAAACGTTGAAAATGATAAACTTCCTCCACCA
CTGCAACCTCAAGTTTATGCAGGATGA
Protein Properties
Number of Residues 708
Molecular Weight 79370.5
Theoretical pI 6.81
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence
>Rab5 GDP/GTP exchange factor
MVVVTGREPDSRRQDGAMSSSDAEDDFLEPATPTATQAGHALPLLPQERCAEFPALRGPP
TQGACSSCVQRGPVLCHRAPPGAAGEHAATEGREGAPSVSGTHALLQRPLGADCGDRPAA
CGPAEGPLCQAQVVSRKKMSLKSERRGIHVDQSDLLCKKGCGYYGNPAWQGFCSKCWREE
YHKARQKQIQEDWELAERVLLCCPGWSAMVQFQLTATSASWAQVILLLQPPKWLGLQKLQ
REEEEAFASSQSSQGAQSLTFSKFEEKKTNEKTRKVTTVKKFFSASSRVGSKKEIQEAKA
PSPSINRQTSIETDRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECA
QDFYHNVAERMQTRGKERRFHHVGQAGLELLTSGDPPASASQSAGNTGVEPPHPAVPPER
VEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPE
VSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIV
LKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDR
YMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTD
GIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG
GenBank ID Protein 34534860
UniProtKB/Swiss-Prot ID Q9UJ41
UniProtKB/Swiss-Prot Entry Name RABX5_HUMAN
PDB IDs Not Available
GenBank Gene ID AK127790
GeneCard ID RABGEF1
GenAtlas ID RABGEF1
HGNC ID HGNC:17676
References
General References
  1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [PubMed:14702039 ]
  2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  3. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M: Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14;325(5942):834-40. doi: 10.1126/science.1175371. Epub 2009 Jul 16. [PubMed:19608861 ]
  4. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [PubMed:18669648 ]
  5. Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Korner R, Greff Z, Keri G, Stemmann O, Mann M: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell. 2008 Aug 8;31(3):438-48. doi: 10.1016/j.molcel.2008.07.007. [PubMed:18691976 ]
  6. Zahedi RP, Lewandrowski U, Wiesner J, Wortelkamp S, Moebius J, Schutz C, Walter U, Gambaryan S, Sickmann A: Phosphoproteome of resting human platelets. J Proteome Res. 2008 Feb;7(2):526-34. Epub 2007 Dec 19. [PubMed:18088087 ]
  7. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [PubMed:19413330 ]
  8. Hillier LW, Fulton RS, Fulton LA, Graves TA, Pepin KH, Wagner-McPherson C, Layman D, Maas J, Jaeger S, Walker R, Wylie K, Sekhon M, Becker MC, O'Laughlin MD, Schaller ME, Fewell GA, Delehaunty KD, Miner TL, Nash WE, Cordes M, Du H, Sun H, Edwards J, Bradshaw-Cordum H, Ali J, Andrews S, Isak A, Vanbrunt A, Nguyen C, Du F, Lamar B, Courtney L, Kalicki J, Ozersky P, Bielicki L, Scott K, Holmes A, Harkins R, Harris A, Strong CM, Hou S, Tomlinson C, Dauphin-Kohlberg S, Kozlowicz-Reilly A, Leonard S, Rohlfing T, Rock SM, Tin-Wollam AM, Abbott A, Minx P, Maupin R, Strowmatt C, Latreille P, Miller N, Johnson D, Murray J, Woessner JP, Wendl MC, Yang SP, Schultz BR, Wallis JW, Spieth J, Bieri TA, Nelson JO, Berkowicz N, Wohldmann PE, Cook LL, Hickenbotham MT, Eldred J, Williams D, Bedell JA, Mardis ER, Clifton SW, Chissoe SL, Marra MA, Raymond C, Haugen E, Gillett W, Zhou Y, James R, Phelps K, Iadanoto S, Bubb K, Simms E, Levy R, Clendenning J, Kaul R, Kent WJ, Furey TS, Baertsch RA, Brent MR, Keibler E, Flicek P, Bork P, Suyama M, Bailey JA, Portnoy ME, Torrents D, Chinwalla AT, Gish WR, Eddy SR, McPherson JD, Olson MV, Eichler EE, Green ED, Waterston RH, Wilson RK: The DNA sequence of human chromosome 7. Nature. 2003 Jul 10;424(6945):157-64. [PubMed:12853948 ]
  9. Mattera R, Arighi CN, Lodge R, Zerial M, Bonifacino JS: Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5 complex. EMBO J. 2003 Jan 2;22(1):78-88. [PubMed:12505986 ]
  10. Horiuchi H, Lippe R, McBride HM, Rubino M, Woodman P, Stenmark H, Rybin V, Wilm M, Ashman K, Mann M, Zerial M: A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function. Cell. 1997 Sep 19;90(6):1149-59. [PubMed:9323142 ]
  11. Lippe R, Miaczynska M, Rybin V, Runge A, Zerial M: Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex. Mol Biol Cell. 2001 Jul;12(7):2219-28. [PubMed:11452015 ]
  12. Nimmrich I, Erdmann S, Melchers U, Finke U, Hentsch S, Moyer MP, Hoffmann I, Muller O: Seven genes that are differentially transcribed in colorectal tumor cell lines. Cancer Lett. 2000 Nov 10;160(1):37-43. [PubMed:11098082 ]
  13. Delprato A, Merithew E, Lambright DG: Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5. Cell. 2004 Sep 3;118(5):607-17. [PubMed:15339665 ]
  14. Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S, Schneider TR: Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. Cell. 2006 Mar 24;124(6):1183-95. Epub 2006 Feb 23. [PubMed:16499958 ]
  15. Delprato A, Lambright DG: Structural basis for Rab GTPase activation by VPS9 domain exchange factors. Nat Struct Mol Biol. 2007 May;14(5):406-12. Epub 2007 Apr 22. [PubMed:17450153 ]