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Showing Protein Zinc finger protein PLAG1 (HMDBP10914)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 1 metabolite
Identification
HMDB Protein ID HMDBP10914
Secondary Accession Numbers
  • 17215
Name Zinc finger protein PLAG1
Synonyms
  1. Pleiomorphic adenoma gene 1 protein
Gene Name PLAG1
Protein Type Unknown
Biological Properties
General Function Involved in zinc ion binding
Specific Function Transcription factor whose activation results in up- regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation:when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia
Pathways Not Available
Reactions Not Available
GO Classification
Component
cell part
intracellular
Function
ion binding
cation binding
metal ion binding
binding
transition metal ion binding
zinc ion binding
nucleic acid binding
Cellular Location
  1. Nucleus
Gene Properties
Chromosome Location Chromosome:8
Locus 8q12
SNPs PLAG1
Gene Sequence 
>1503 bp
ATGGCCACTGTCATTCCTGGTGATTTGTCAGAAGTAAGAGATACCCAGAAAGTCCCTTCA
GGGAAACGTAAGCGTGGTGAAACCAAACCAAGAAAAAACTTTCCTTGCCAACTGTGTGAC
AAGGCCTTTAACAGTGTTGAGAAATTAAAGGTTCACTCCTACTCTCACACAGGAGAGAGG
CCCTACAAGTGCATACAACAAGACTGCACCAAGGCCTTTGTTTCTAAGTACAAATTACAA
AGGCACATGGCTACTCATTCTCCTGAGAAAACCCACAAGTGTAATTATTGTGAGAAAATG
TTTCACCGGAAAGATCATCTGAAGAATCACCTCCATACACACGACCCTAACAAAGAGACG
TTTAAGTGCGAAGAATGTGGCAAGAACTACAATACCAAGCTTGGATTTAAACGTCACTTG
GCCTTGCATGCCGCAACAAGTGGTGACCTCACCTGTAAGGTATGTTTGCAAACTTTTGAA
AGCACGGGAGTGCTTCTGGAGCACCTTAAATCTCATGCAGGCAAGTCGTCTGGTGGGGTT
AAAGAAAAAAAGCACCAGTGCGAACATTGTGATCGCCGGTTCTACACCCGAAAGGATGTC
CGGAGACACATGGTGGTGCACACTGGAAGAAAGGACTTCCTCTGTCAGTATTGTGCACAG
AGATTTGGGCGAAAGGATCACCTGACTCGACATATGAAGAAGAGTCACAATCAAGAGCTT
CTGAAGGTCAAAACAGAACCAGTGGATTTCCTTGACCCATTTACCTGCAATGTGTCTGTG
CCTATAAAAGACGAGCTCCTTCCGGTGATGTCCTTACCTTCCAGTGAACTGTTATCAAAG
CCATTCACAAACACTTTGCAGTTAAACCTCTACAACACTCCATTTCAGTCCATGCAGAGC
TCGGGATCTGCCCACCAAATGATCACAACTTTACCTTTGGGAATGACATGCCCAATAGAT
ATGGACACTGTTCATCCCTCTCACCACCTTTCTTTCAAATATCCGTTCAGTTCTACCTCA
TATGCAATTTCTATTCCTGAAAAAGAACAGCCATTAAAGGGGGAAATTGAGAGTTACCTG
ATGGAGTTACAAGGTGGCGTGCCCTCTTCATCCCAAGATTCTCAAGCATCGTCATCATCT
AAGCTAGGGTTGGATCCTCAGATTGGGTCCCTAGATGATGGTGCAGGAGACCTCTCCCTA
TCCAAAAGCTCTATCTCCATCAGTGACCCCCTAAACACACCAGCATTGGATTTTTCTCAG
TTGTTTAATTTCATACCTTTAAATGGTCCTCCCTATAATCCTCTATCAGTGGGGAGCCTT
GGAATGAGCTATTCCCAGGAAGAAGCACATTCTTCTGTTTCCCAGCTCCCCCCACAAACA
CAGGATCTTCAGGATCCTGCAAACACTATAGGGCTTGGGTCTCTGCACTCACTGTCAGCA
GCTTTCACCAGCAGTTTAAGCACAAGTACCACCCTCCCACGTTTCCATCAAGCTTTTCAG
TAG
Protein Properties
Number of Residues 500
Molecular Weight 55909.0
Theoretical pI 8.7
Pfam Domain Function Not Available
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence 
>Zinc finger protein PLAG1
MATVIPGDLSEVRDTQKVPSGKRKRGETKPRKNFPCQLCDKAFNSVEKLKVHSYSHTGER
PYKCIQQDCTKAFVSKYKLQRHMATHSPEKTHKCNYCEKMFHRKDHLKNHLHTHDPNKET
FKCEECGKNYNTKLGFKRHLALHAATSGDLTCKVCLQTFESTGVLLEHLKSHAGKSSGGV
KEKKHQCEHCDRRFYTRKDVRRHMVVHTGRKDFLCQYCAQRFGRKDHLTRHMKKSHNQEL
LKVKTEPVDFLDPFTCNVSVPIKDELLPVMSLPSSELLSKPFTNTLQLNLYNTPFQSMQS
SGSAHQMITTLPLGMTCPIDMDTVHPSHHLSFKYPFSSTSYAISIPEKEQPLKGEIESYL
MELQGGVPSSSQDSQASSSSKLGLDPQIGSLDDGAGDLSLSKSSISISDPLNTPALDFSQ
LFNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPPQTQDLQDPANTIGLGSLHSLSA
AFTSSLSTSTTLPRFHQAFQ
GenBank ID Protein 167857796
UniProtKB/Swiss-Prot ID Q6DJT9
UniProtKB/Swiss-Prot Entry Name PLAG1_HUMAN
PDB IDs Not Available
GenBank Gene ID NM_001114634.1
GeneCard ID PLAG1
GenAtlas ID PLAG1
HGNC ID HGNC:9045
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Hibbard MK, Kozakewich HP, Dal Cin P, Sciot R, Tan X, Xiao S, Fletcher JA: PLAG1 fusion oncogenes in lipoblastoma. Cancer Res. 2000 Sep 1;60(17):4869-72. [PubMed:10987300 ]
  3. Van Dyck F, Delvaux EL, Van de Ven WJ, Chavez MV: Repression of the Transactivating Capacity of the Oncoprotein PLAG1 by SUMOylation. J Biol Chem. 2004 Aug 20;279(34):36121-31. Epub 2004 Jun 18. [PubMed:15208321 ]
  4. Morerio C, Rapella A, Rosanda C, Tassano E, Gambini C, Romagnoli G, Panarello C: PLAG1-HAS2 fusion in lipoblastoma with masked 8q intrachromosomal rearrangement. Cancer Genet Cytogenet. 2005 Jan 15;156(2):183-4. [PubMed:15642402 ]
  5. Kas K, Voz ML, Roijer E, Astrom AK, Meyen E, Stenman G, Van de Ven WJ: Promoter swapping between the genes for a novel zinc finger protein and beta-catenin in pleiomorphic adenomas with t(3;8)(p21;q12) translocations. Nat Genet. 1997 Feb;15(2):170-4. [PubMed:9020842 ]
  6. Astrom AK, Voz ML, Kas K, Roijer E, Wedell B, Mandahl N, Van de Ven W, Mark J, Stenman G: Conserved mechanism of PLAG1 activation in salivary gland tumors with and without chromosome 8q12 abnormalities: identification of SII as a new fusion partner gene. Cancer Res. 1999 Feb 15;59(4):918-23. [PubMed:10029085 ]
  7. Braem CV, Kas K, Meyen E, Debiec-Rychter M, Van De Ven WJ, Voz ML: Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal. J Biol Chem. 2002 May 31;277(22):19673-8. Epub 2002 Mar 6. [PubMed:11882654 ]
  8. Kas K, Voz ML, Hensen K, Meyen E, Van de Ven WJ: Transcriptional activation capacity of the novel PLAG family of zinc finger proteins. J Biol Chem. 1998 Sep 4;273(36):23026-32. [PubMed:9722527 ]
  9. Voz ML, Astrom AK, Kas K, Mark J, Stenman G, Van de Ven WJ: The recurrent translocation t(5;8)(p13;q12) in pleomorphic adenomas results in upregulation of PLAG1 gene expression under control of the LIFR promoter. Oncogene. 1998 Mar;16(11):1409-16. [PubMed:9525740 ]
  10. Voz ML, Agten NS, Van de Ven WJ, Kas K: PLAG1, the main translocation target in pleomorphic adenoma of the salivary glands, is a positive regulator of IGF-II. Cancer Res. 2000 Jan 1;60(1):106-13. [PubMed:10646861 ]
  11. Hensen K, Van Valckenborgh IC, Kas K, Van de Ven WJ, Voz ML: The tumorigenic diversity of the three PLAG family members is associated with different DNA binding capacities. Cancer Res. 2002 Mar 1;62(5):1510-7. [PubMed:11888928 ]
  12. Zatkova A, Rouillard JM, Hartmann W, Lamb BJ, Kuick R, Eckart M, von Schweinitz D, Koch A, Fonatsch C, Pietsch T, Hanash SM, Wimmer K: Amplification and overexpression of the IGF2 regulator PLAG1 in hepatoblastoma. Genes Chromosomes Cancer. 2004 Feb;39(2):126-37. [PubMed:14695992 ]
  13. Voz ML, Mathys J, Hensen K, Pendeville H, Van Valckenborgh I, Van Huffel C, Chavez M, Van Damme B, De Moor B, Moreau Y, Van de Ven WJ: Microarray screening for target genes of the proto-oncogene PLAG1. Oncogene. 2004 Jan 8;23(1):179-91. [PubMed:14712223 ]
  14. Zheng G, Yang YC: Sumoylation and acetylation play opposite roles in the transactivation of PLAG1 and PLAGL2. J Biol Chem. 2005 Dec 9;280(49):40773-81. Epub 2005 Oct 5. [PubMed:16207715 ]
  15. Asp J, Persson F, Kost-Alimova M, Stenman G: CHCHD7-PLAG1 and TCEA1-PLAG1 gene fusions resulting from cryptic, intrachromosomal 8q rearrangements in pleomorphic salivary gland adenomas. Genes Chromosomes Cancer. 2006 Sep;45(9):820-8. [PubMed:16736500 ]