Human Metabolome Database: Showing Protein Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 (HMDBP11864)

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Identification Biological properties Gene properties Protein properties External links References XML Show 4 metabolites

Identification

HMDB Protein ID

HMDBP11864

Secondary Accession Numbers

None

Name

Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66

Synonyms

60S ribosomal protein L8 histidine hydroxylase
Histone lysine demethylase NO66
Myc-associated protein with JmjC domain
Nucleolar protein 66
Ribosomal oxygenase NO66
hsNO66
ROX

Gene Name

NO66

Protein Type

Unknown

Biological Properties

General Function

Not Available

Specific Function

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.

Pathways

Not Available

Reactions

Protein N(6),N(6)-dimethyl-L-lysine + Oxoglutaric acid + Oxygen → protein N(6)-methyl-L-lysine + Succinic acid + Formaldehyde + CO(2)	details
Protein N(6)-methyl-L-lysine + Oxoglutaric acid + Oxygen → protein L-lysine + Succinic acid + Formaldehyde + CO(2)	details
L-histidine-[60S ribosomal protein L8] + Oxoglutaric acid + Oxygen → (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L8] + Succinic acid + CO(2)	details

GO Classification

Biological Process
negative regulation of transcription, DNA-dependent
negative regulation of osteoblast differentiation
transcription, DNA-dependent
Cellular Component
nucleolus
nucleus
nucleoplasm
Molecular Function
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
iron ion binding
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-K36 specific)

Cellular Location

Not Available

Gene Properties

Chromosome Location

Locus

14q24.3

SNPs

Not Available

Gene Sequence

Not Available

Protein Properties

Number of Residues

Not Available

Molecular Weight

71084.97

Theoretical pI

6.455

Pfam Domain Function

Not Available

Signals

Not Available

Transmembrane Regions

Not Available

Protein Sequence

>>gi|106879206|ref|NP_078920.2| bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 [Homo sapiens]
MDGLQASAGPLRRGRPKRRRKPQPHSGSVLALPLRSRKIRKQLRSVVSRMAALRTQTLPS
ENSEESRVES

External Links

GenBank ID Protein

Not Available

UniProtKB/Swiss-Prot ID

Q9H6W3

UniProtKB/Swiss-Prot Entry Name

Not Available

PDB IDs