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Showing Protein Histone-lysine N-methyltransferase PRDM16 (HMDBP14501)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 1 metabolite
Identification
HMDB Protein ID HMDBP14501
Secondary Accession Numbers None
Name Histone-lysine N-methyltransferase PRDM16
Synonyms
  1. PR domain zinc finger protein 16
  2. PR domain-containing protein 16
  3. Transcription factor MEL1
  4. MDS1/EVI1-like gene 1
Gene Name PRDM16
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Binds DNA and functions as a transcriptional regulator (PubMed:12816872). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (By similarity). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (By similarity). Functions as a repressor of TGF-beta signaling (PubMed:19049980).Binds DNA and functions as a transcriptional regulator (PubMed:12816872). Functions as a repressor of TGF-beta signaling (PubMed:14656887). May regulate granulocyte differentiation (PubMed:12816872).
Pathways
  • Lysine degradation
  • Thermogenesis
Reactions Not Available
GO Classification
Biological Process
negative regulation of granulocyte differentiation
heterochromatin organization
brown fat cell differentiation
regulation of transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-dependent
positive regulation of transcription, DNA-dependent
negative regulation of transcription from RNA polymerase II promoter
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of cellular respiration
positive regulation of cold-induced thermogenesis
Cellular Component
cytosol
aggresome
nucleus
nucleoplasm
transcriptional repressor complex
Molecular Function
histone methyltransferase activity (H3-K9 specific)
metal ion binding
DNA-binding transcription repressor activity, RNA polymerase II-specific
sequence-specific DNA binding
DNA-binding transcription activator activity, RNA polymerase II-specific
transcription coactivator activity
activating transcription factor binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription regulatory region sequence-specific DNA binding
transcription cofactor activity
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues 1276
Molecular Weight 140250.085
Theoretical pI 6.217
Pfam Domain Function
Signals Not Available
Transmembrane Regions Not Available
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q9HAZ2
UniProtKB/Swiss-Prot Entry Name PRD16_HUMAN
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
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  3. Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T: Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. DNA Res. 2002 Jun 30;9(3):99-106. [PubMed:12168954 ]
  4. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O: Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 2000 Dec 31;7(6):347-55. [PubMed:11214970 ]
  5. Takahata M, Inoue Y, Tsuda H, Imoto I, Koinuma D, Hayashi M, Ichikura T, Yamori T, Nagasaki K, Yoshida M, Matsuoka M, Morishita K, Yuki K, Hanyu A, Miyazawa K, Inazawa J, Miyazono K, Imamura T: SKI and MEL1 cooperate to inhibit transforming growth factor-beta signal in gastric cancer cells. J Biol Chem. 2009 Jan 30;284(5):3334-44. doi: 10.1074/jbc.M808989200. Epub 2008 Dec 1. [PubMed:19049980 ]
  6. Mochizuki N, Shimizu S, Nagasawa T, Tanaka H, Taniwaki M, Yokota J, Morishita K: A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood. 2000 Nov 1;96(9):3209-14. [PubMed:11050005 ]
  7. Nishikata I, Sasaki H, Iga M, Tateno Y, Imayoshi S, Asou N, Nakamura T, Morishita K: A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is expressed mainly in t(1;3)(p36;q21)-positive AML and blocks G-CSF-induced myeloid differentiation. Blood. 2003 Nov 1;102(9):3323-32. doi: 10.1182/blood-2002-12-3944. Epub 2003 Jun 19. [PubMed:12816872 ]
  8. Xinh PT, Tri NK, Nagao H, Nakazato H, Taketazu F, Fujisawa S, Yagasaki F, Chen YZ, Hayashi Y, Toyoda A, Hattori M, Sakaki Y, Tokunaga K, Sato Y: Breakpoints at 1p36.3 in three MDS/AML(M4) patients with t(1;3)(p36;q21) occur in the first intron and in the 5' region of MEL1. Genes Chromosomes Cancer. 2003 Mar;36(3):313-6. doi: 10.1002/gcc.10176. [PubMed:12557231 ]
  9. Yoshida M, Nosaka K, Yasunaga J, Nishikata I, Morishita K, Matsuoka M: Aberrant expression of the MEL1S gene identified in association with hypomethylation in adult T-cell leukemia cells. Blood. 2004 Apr 1;103(7):2753-60. doi: 10.1182/blood-2003-07-2482. Epub 2003 Dec 4. [PubMed:14656887 ]
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