Hmdb loader

Showing Protein Na(+)/H(+) exchange regulatory cofactor NHE-RF3 (HMDBP08224)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 1 metabolite
Identification
HMDB Protein ID HMDBP08224
Secondary Accession Numbers
  • 13936
Name Na(+)/H(+) exchange regulatory cofactor NHE-RF3
Synonyms
  1. CFTR-associated protein of 70 kDa
  2. NHERF-3
  3. Na(+)/H(+) exchanger regulatory factor 3
  4. Na/Pi cotransporter C-terminal-associated protein 1
  5. NaPi-Cap1
  6. PDZ domain-containing protein 1
  7. Sodium-hydrogen exchanger regulatory factor 3
Gene Name PDZK1
Protein Type Unknown
Biological Properties
General Function Involved in protein binding
Specific Function A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function
Pathways Not Available
Reactions Not Available
GO Classification
Function
binding
protein binding
Cellular Location
  1. Cytoplasm
  2. Peripheral membrane protein
  3. Membrane
Gene Properties
Chromosome Location Chromosome:1
Locus 1q21
SNPs PDZK1
Gene Sequence 
>1560 bp
ATGACCTCCACCTTCAACCCCCGAGAATGTAAACTGTCCAAGCAAGAAGGGCAAAACTAT
GGCTTCTTCCTGCGAATTGAGAAGGACACCGAGGGCCACCTGGTCCGGGTGGTTGAGAAG
TGTAGCCCAGCAGAGAAGGCTGGCCTTCAAGATGGAGACAGAGTTCTTAGGATCAATGGT
GTCTTTGTGGACAAAGAAGAACATATGCAGGTTGTGGATCTGGTCAGAAAGAGTGGGAAT
TCAGTGACTTTACTAGTTCTGGATGGGGATTCCTATGAGAAAGCAGTGAAAACACGGGTG
GACTTGAAAGAGTTGGGTCAAAGTCAGAAGGAGCAAGGTTTGAGTGATAATATACTTTCC
CCTGTGATGAATGGAGGTGTGCAAACTTGGACCCAGCCCCGGCTCTGCTATCTCGTGAAG
GAAGGAGGCAGCTATGGCTTCTCTCTGAAAACTGTCCAAGGTAAAAAGGGGGTGTACATG
ACTGATATTACACCTCAAGGTGTGGCTATGAGAGCTGGAGTTCTGGCTGATGATCACTTG
ATTGAAGTGAATGGAGAGAATGTAGAGGATGCCAGCCATGAGAAAGTGGTTGAAAAGGTG
AAGAAGTCAGGAAGCCGTGTCATGTTCCTGCTGGTGGACAAAGAAACTGACAAGCGTCAT
GTTGAGCAGAAGATACAATTCAAAAGAGAAACAGCCAGTTTGAAACTGTTACCCCACCAG
CCCCGAATTGTGGAGATGAAGAAAGGAAGCAATGGCTATGGTTTCTATCTGAGGGCAGGC
TCAGAACAGAAAGGTCAAATCATCAAGGACATAGATTCTGGAAGTCCAGCAGAGGAGGCT
GGCTTGAAGAACAATGATCTGGTAGTTGCTGTCAACGGCGAGTCTGTGGAAACCCTGGAT
CATGACAGTGTGGTAGAAATGATTAGAAAGGGTGGAGATCAGACTTCACTGTTGGTGGTA
GACAAAGAGACGGACAACATGTACAGACTGGCTCATTTTTCTCCATTTCTCTACTATCAA
AGTCAAGAACTGCCCAATGGCTCTGTCAAGGAGGCTCCAGCTCCTACTCCCACTTCTCTG
GAAGTCTCAAGTCCACCAGATACTACAGAGGAAGTAGATCATAAGCCTAAACTCTGCAGG
CTGGCTAAAGGTGAAAATGGCTATGGCTTTCACTTAAATGCGATTCGGGGTCTGCCAGGC
TCATTCATCAAAGAGGTACAGAAGGGCGGTCCTGCTGACTTGGCTGGGCTAGAGGATGAG
GATGTCATCATTGAAGTGAATGGGGTGAATGTGCTAGATGAACCCTATGAGAAGGTGGTG
GATAGAATCCAGAGCAGTGGGAAGAATGTCACACTTCTAGTCTGTGGAAAGAAGGCCTAT
GATTATTTCCAAGCTAAGAAAATCCCTATTGTTTCCTCCCTGGCTGATCCACTTGACACC
CCTCCAGATTCTAAAGAAGGAATAGTGGTGGAGTCAAACCATGACTCGCACATGGCAAAA
GAACGGGCCCACAGTACAGCCTCACATTCTTCTTCCAATTCTGAAGATACAGAGATGTGA
Protein Properties
Number of Residues 519
Molecular Weight 57129.0
Theoretical pI 5.24
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence 
>Na(+)/H(+) exchange regulatory cofactor NHE-RF3
MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRING
VFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILS
PVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHL
IEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQ
PRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLD
HDSVVEMIRKGGDQTSLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVKEAPAPTPTSL
EVSSPPDTTEEVDHKPKLCRLAKGENGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDE
DVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVCGKKAYDYFQAKKIPIVSSLADPLDT
PPDSKEGIVVESNHDSHMAKERAHSTASHSSSNSEDTEM
GenBank ID Protein 2944189
UniProtKB/Swiss-Prot ID Q5T2W1
UniProtKB/Swiss-Prot Entry Name NHRF3_HUMAN
PDB IDs Not Available
GenBank Gene ID AF012281
GeneCard ID PDZK1
GenAtlas ID PDZK1
HGNC ID HGNC:8821
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke G, Clee C, Cobley V, Collier RE, Corby N, Coville GJ, Davies J, Deadman R, Dunn M, Earthrowl M, Ellington AG, Errington H, Frankish A, Frankland J, French L, Garner P, Garnett J, Gay L, Ghori MR, Gibson R, Gilby LM, Gillett W, Glithero RJ, Grafham DV, Griffiths C, Griffiths-Jones S, Grocock R, Hammond S, Harrison ES, Hart E, Haugen E, Heath PD, Holmes S, Holt K, Howden PJ, Hunt AR, Hunt SE, Hunter G, Isherwood J, James R, Johnson C, Johnson D, Joy A, Kay M, Kershaw JK, Kibukawa M, Kimberley AM, King A, Knights AJ, Lad H, Laird G, Lawlor S, Leongamornlert DA, Lloyd DM, Loveland J, Lovell J, Lush MJ, Lyne R, Martin S, Mashreghi-Mohammadi M, Matthews L, Matthews NS, McLaren S, Milne S, Mistry S, Moore MJ, Nickerson T, O'Dell CN, Oliver K, Palmeiri A, Palmer SA, Parker A, Patel D, Pearce AV, Peck AI, Pelan S, Phelps K, Phillimore BJ, Plumb R, Rajan J, Raymond C, Rouse G, Saenphimmachak C, Sehra HK, Sheridan E, Shownkeen R, Sims S, Skuce CD, Smith M, Steward C, Subramanian S, Sycamore N, Tracey A, Tromans A, Van Helmond Z, Wall M, Wallis JM, White S, Whitehead SL, Wilkinson JE, Willey DL, Williams H, Wilming L, Wray PW, Wu Z, Coulson A, Vaudin M, Sulston JE, Durbin R, Hubbard T, Wooster R, Dunham I, Carter NP, McVean G, Ross MT, Harrow J, Olson MV, Beck S, Rogers J, Bentley DR, Banerjee R, Bryant SP, Burford DC, Burrill WD, Clegg SM, Dhami P, Dovey O, Faulkner LM, Gribble SM, Langford CF, Pandian RD, Porter KM, Prigmore E: The DNA sequence and biological annotation of human chromosome 1. Nature. 2006 May 18;441(7091):315-21. [PubMed:16710414 ]
  3. Malmberg EK, Andersson CX, Gentzsch M, Chen JH, Mengos A, Cui L, Hansson GC, Riordan JR: Bcr (breakpoint cluster region) protein binds to PDZ-domains of scaffold protein PDZK1 and vesicle coat protein Mint3. J Cell Sci. 2004 Nov 1;117(Pt 23):5535-41. Epub 2004 Oct 19. [PubMed:15494376 ]
  4. Rossmann H, Jacob P, Baisch S, Hassoun R, Meier J, Natour D, Yahya K, Yun C, Biber J, Lackner KJ, Fiehn W, Gregor M, Seidler U, Lamprecht G: The CFTR associated protein CAP70 interacts with the apical Cl-/HCO3- exchanger DRA in rabbit small intestinal mucosa. Biochemistry. 2005 Mar 22;44(11):4477-87. [PubMed:15766278 ]
  5. Kocher O, Comella N, Tognazzi K, Brown LF: Identification and partial characterization of PDZK1: a novel protein containing PDZ interaction domains. Lab Invest. 1998 Jan;78(1):117-25. [PubMed:9461128 ]
  6. Kocher O, Comella N, Gilchrist A, Pal R, Tognazzi K, Brown LF, Knoll JH: PDZK1, a novel PDZ domain-containing protein up-regulated in carcinomas and mapped to chromosome 1q21, interacts with cMOAT (MRP2), the multidrug resistance-associated protein. Lab Invest. 1999 Sep;79(9):1161-70. [PubMed:10496535 ]
  7. Wang S, Yue H, Derin RB, Guggino WB, Li M: Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity. Cell. 2000 Sep 29;103(1):169-79. [PubMed:11051556 ]