| Identification |
|---|
| HMDB Protein ID
| HMDBP12178 |
| Secondary Accession Numbers
| None |
| Name
| Polyunsaturated fatty acid lipoxygenase ALOX15B |
| Synonyms
|
- 15-lipoxygenase 2
- Arachidonate 15-lipoxygenase B
- Arachidonate 15-lipoxygenase type II
- Linoleate 13-lipoxygenase 15-LOb
- 15-LOX-2
- 15-LOX-B
|
| Gene Name
| ALOX15B |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (PubMed:23382512). Inserts a peroxyl group at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (PubMed:23382512). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (PubMed:23382512). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist.Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets. In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids. Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells (By similarity). |
| Pathways
|
- Arachidonic acid metabolism
- hydroperoxy eicosatetraenoic acid biosynthesis
- Serotonergic synapse
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| negative regulation of cell cycle |
| cannabinoid biosynthetic process |
| endocannabinoid signaling pathway |
| hepoxilin biosynthetic process |
| lipoxin A4 biosynthetic process |
| positive regulation of chemokine production |
| phospholipid metabolic process |
| positive regulation of macrophage derived foam cell differentiation |
| positive regulation of peroxisome proliferator activated receptor signaling pathway |
| negative regulation of growth |
| lipoxygenase pathway |
| positive regulation of keratinocyte differentiation |
| lipid oxidation |
| linoleic acid metabolic process |
| arachidonic acid metabolic process |
| negative regulation of cell proliferation |
| lipid metabolic process |
| Cellular Component |
| cytosol |
| cytoskeleton |
| focal adhesion |
| plasma membrane |
| nucleus |
| adherens junction |
| extrinsic to membrane |
| membrane |
| Molecular Function |
| arachidonate 8(S)-lipoxygenase activity |
| linoleate 9S-lipoxygenase activity |
| lipid binding |
| oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen |
| lipoxygenase activity |
| arachidonate 15-lipoxygenase activity |
| iron ion binding |
| calcium ion binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 677 |
| Molecular Weight
| 76144.58 |
| Theoretical pI
| 6.282 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| Q8K4F2 |
| UniProtKB/Swiss-Prot Entry Name
| LX15B_RAT |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Gregus AM, Dumlao DS, Wei SC, Norris PC, Catella LC, Meyerstein FG, Buczynski MW, Steinauer JJ, Fitzsimmons BL, Yaksh TL, Dennis EA: Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia. FASEB J. 2013 May;27(5):1939-49. doi: 10.1096/fj.12-217414. Epub 2013 Feb 4. [PubMed:23382512 ]
|
