| Identification |
|---|
| HMDB Protein ID
| HMDBP14314 |
| Secondary Accession Numbers
| None |
| Name
| Genome polyprotein |
| Synonyms
|
Not Available
|
| Gene Name
| Not Available |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions. Overcomes the anti-viral effects of host EXOC1 by sequestering and degrading the latter through the proteasome degradation pathway.Inhibits RNA silencing by interfering with host Dicer.Prevents premature fusion activity of envelope proteins in trans-Golgi by binding to envelope protein E at pH6.0. After virion release in extracellular space, gets dissociated from E dimers.Acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is the only viral peptide matured by host furin in the trans-Golgi network probably to avoid catastrophic activation of the viral fusion activity in acidic Golgi compartment prior to virion release. prM-E cleavage is inefficient, and many virions are only partially matured. These uncleaved prM would play a role in immune evasion.May play a role in virus budding. Exerts cytotoxic effects by activating a mitochondrial apoptotic pathway through M ectodomain. May display a viroporin activity.Binds to host cell surface receptor and mediates fusion between viral and cellular membranes. Envelope protein is synthesized in the endoplasmic reticulum in the form of heterodimer with protein prM. They play a role in virion budding in the ER, and the newly formed immature particle is covered with 60 spikes composed of heterodimer between precursor prM and envelope protein E. The virion is transported to the Golgi apparatus where the low pH causes dissociation of PrM-E heterodimers and formation of E homodimers. prM-E cleavage is inefficient, and many virions are only partially matured. These uncleaved prM would play a role in immune evasion.Involved in immune evasion, pathogenesis and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. Essential for viral replication. Required for formation of the replication complex and recruitment of other non-structural proteins to the ER-derived membrane structures. Excreted as a hexameric lipoparticle that plays a role against host immune response. Antagonizing the complement function. Binds to the host macrophages and dendritic cells. Inhibits signal transduction originating from Toll-like receptor 3 (TLR3).Disrupts the host endothelial glycocalyx layer of host pulmonary microvascular endothelial cells, inducing degradation of sialic acid and shedding of heparan sulfate proteoglycans. NS1 induces expression of sialidases, heparanase, and activates cathepsin L, which activates heparanase via enzymatic cleavage. These effects are probably linked to the endothelial hyperpermeability observed in severe dengue disease.Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.Required cofactor for the serine protease function of NS3. May have membrane-destabilizing activity and form viroporins (By similarity).Displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction.Regulates the ATPase activity of the NS3 helicase activity. NS4A allows NS3 helicase to conserve energy during unwinding. Plays a role in the inhibition of the host innate immune response. Interacts with host MAVS and thereby prevents the interaction between DDX58 and MAVS. In turn, IFN-beta production is impaired. Interacts with host AUP1 which mediates induction of lipophagy in host cells and facilitates production of virus progeny particles (By similarity).Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.Induces the formation of ER-derived membrane vesicles where the viral replication takes place. Inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway.Replicates the viral (+) and (-) RNA genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in RNA genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host TYK2 and STAT2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway (By similarity). May reduce immune responses by preventing the recruitment of the host PAF1 complex to interferon-responsive genes (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| clathrin-dependent endocytosis of virus by host cell |
| induction by virus of host autophagy |
| pore formation by virus in membrane of host cell |
| suppression by virus of host MAVS activity |
| suppression by virus of host STAT2 activity |
| suppression by virus of host TYK2 activity |
| virion attachment to host cell |
| protein oligomerization |
| viral RNA genome replication |
| suppression by virus of host type I interferon-mediated signaling pathway |
| fusion of virus membrane with host endosome membrane |
| Cellular Component |
| host cell mitochondrion |
| host cell perinuclear region of cytoplasm |
| integral to membrane of host cell |
| extracellular region |
| virion membrane |
| host cell nucleus |
| viral envelope |
| viral capsid |
| host cell endoplasmic reticulum membrane |
| integral to membrane |
| Molecular Function |
| RNA-directed RNA polymerase activity |
| ion channel activity |
| metal ion binding |
| RNA helicase activity |
| mRNA (nucleoside-2'-O-)-methyltransferase activity |
| nucleoside-triphosphatase activity |
| double-stranded RNA binding |
| ATP binding |
| mRNA (guanine-N7-)-methyltransferase activity |
| structural molecule activity |
| serine-type endopeptidase activity |
| protein dimerization activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 379497.86 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
- 102-122;239-259;266-280;726-746;753-773;1196-1220;1227-1245;1270-1290;1292-1310;1318-1338;1347-1367;1371-1391;2148-2168;2193-2213;2229-2249;2348-2368;2414-2434;2460-2480;
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P14340 |
| UniProtKB/Swiss-Prot Entry Name
| POLG_DEN2N |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Irie K, Mohan PM, Sasaguri Y, Putnak R, Padmanabhan R: Sequence analysis of cloned dengue virus type 2 genome (New Guinea-C strain). Gene. 1989 Feb 20;75(2):197-211. [PubMed:2714651 ]
- Putnak JR, Charles PC, Padmanabhan R, Irie K, Hoke CH, Burke DS: Functional and antigenic domains of the dengue-2 virus nonstructural glycoprotein NS-1. Virology. 1988 Mar;163(1):93-103. [PubMed:2964755 ]
- Kapoor M, Zhang L, Ramachandra M, Kusukawa J, Ebner KE, Padmanabhan R: Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5. J Biol Chem. 1995 Aug 11;270(32):19100-6. [PubMed:7642575 ]
- Miller S, Sparacio S, Bartenschlager R: Subcellular localization and membrane topology of the Dengue virus type 2 Non-structural protein 4B. J Biol Chem. 2006 Mar 31;281(13):8854-63. Epub 2006 Jan 25. [PubMed:16436383 ]
- Yon C, Teramoto T, Mueller N, Phelan J, Ganesh VK, Murthy KH, Padmanabhan R: Modulation of the nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase. J Biol Chem. 2005 Jul 22;280(29):27412-9. doi: 10.1074/jbc.M501393200. Epub 2005 May 24. [PubMed:15917225 ]
- Miller S, Kastner S, Krijnse-Locker J, Buhler S, Bartenschlager R: The non-structural protein 4A of dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner. J Biol Chem. 2007 Mar 23;282(12):8873-82. doi: 10.1074/jbc.M609919200. Epub 2007 Feb 2. [PubMed:17276984 ]
- Xie X, Gayen S, Kang C, Yuan Z, Shi PY: Membrane topology and function of dengue virus NS2A protein. J Virol. 2013 Apr;87(8):4609-22. doi: 10.1128/JVI.02424-12. Epub 2013 Feb 13. [PubMed:23408612 ]
- Biedrzycka A, Cauchi MR, Bartholomeusz A, Gorman JJ, Wright PJ: Characterization of protease cleavage sites involved in the formation of the envelope glycoprotein and three non-structural proteins of dengue virus type 2, New Guinea C strain. J Gen Virol. 1987 May;68 ( Pt 5):1317-26. doi: 10.1099/0022-1317-68-5-1317. [PubMed:2952760 ]
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